Construction of HGF-Displaying Yeast by Cell Surface Engineering

Overview

Journal Microorganisms

Specialty Microbiology

Date 2022 Jul 27

PMID 35889092

Authors

Seiji Shibasaki

Yuki Nakatani

Kazuaki Taketani

Miki Karasaki

Kiyoshi Matsui

Mitsuyoshi Ueda

Tsuyoshi Iwasaki

Affiliations

Soon will be listed here.

Abstract

Hepatocyte growth factor (HGF) has been investigated as a regulator for immune reactions caused by transplantation and autoimmune diseases and other biological functions. Previous studies demonstrated that cDNA-encoding HGF administration could inhibit acute graft-versus-host disease (GVHD) after treatment via hematopoietic stem cell transplantation. This study aimed to show the preparation of HGF protein on yeast cell surfaces to develop a tool for the oral administration of HGF to a GVHD mouse model. In this study, full-length HGF and the heavy chain of HGF were genetically fused with α-agglutinin and were successfully displayed on the yeast cell surface. This study suggested that yeast cell surface display engineering could provide a novel administration route for HGF.

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