High-Throughput Microbial Community Analyses to Establish a Natural Fungal and Bacterial Consortium from Sewage Sludge Enriched with Three Pharmaceutical Compounds

Overview

Journal J Fungi (Basel)

Date 2022 Jul 27

PMID 35887425

Authors

Alejandro Ledezma-Villanueva

Tatiana Robledo-Mahon

Cinta Gomez-Silvan

Gabriela Angeles-De Paz

Clementina Pozo

Maximino Manzanera

Concepcion Calvo

Elisabet Aranda

Affiliations

Soon will be listed here.

Abstract

Emerging and unregulated contaminants end up in soils via stabilized/composted sewage sludges, paired with possible risks associated with the development of microbial resistance to antimicrobial agents or an imbalance in the microbial communities. An enrichment experiment was performed, fortifying the sewage sludge with carbamazepine, ketoprofen and diclofenac as model compounds, with the aim to obtain strains with the capability to transform these pollutants. Culturable microorganisms were obtained at the end of the experiment. Among fungi, , and showed remarkable degradation rates. Population shifts in bacterial and fungal communities were also studied during the selective pressure using Illumina MiSeq. These analyses showed a predominance of Ascomycota ( and ) and and , suggesting the possibility of selecting native microorganisms to carry out bioremediation processes using tailored techniques.

Citing Articles

Optimizing Bioaugmentation for Pharmaceutical Stabilization of Sewage Sludge: A Study on Short-Term Composting Under Real Conditions.

Angeles-De Paz G, Cubero-Cardoso J, Pozo C, Calvo C, Aranda E, Robledo-Mahon T J Fungi (Basel). 2025; 11(1).

PMID: 39852486 PMC: 11766644. DOI: 10.3390/jof11010067.

Ketoprofen as an emerging contaminant: occurrence, ecotoxicity and (bio)removal.

Tyumina E, Subbotina M, Polygalov M, Tyan S, Ivshina I Front Microbiol. 2023; 14:1200108.

PMID: 37608946 PMC: 10441242. DOI: 10.3389/fmicb.2023.1200108.

Progressive Biocatalysts for the Treatment of Aqueous Systems Containing Pharmaceutical Pollutants.

Efremenko E, Stepanov N, Senko O, Maslova O, Lyagin I, Aslanli A Life (Basel). 2023; 13(3).

PMID: 36983996 PMC: 10052509. DOI: 10.3390/life13030841.

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