Effect of Pre-Induced Mesenchymal Stem Cell-Coated Cellulose/Collagen Nanofibrous Nerve Conduit on Regeneration of Transected Facial Nerve

Overview

Journal Int J Mol Sci

Publisher MDPI

Specialties Biochemistry
Chemistry
Molecular Biology

Date 2022 Jul 27

PMID 35886987

Authors

GwangWon Cho

Changjong Moon

Nagarajan Maharajan

Mary Jasmin Ang

Minseong Kim

Chul Ho Jang

Affiliations

Soon will be listed here.

Abstract

(1) Objective: In order to evaluate the effect of a pre-induced mesenchymal stem cell (MSC)-coated cellulose/collagen nanofibrous nerve conduit on facial nerve regeneration in a rat model both in vitro and in vivo. (2) Methods: After fabrication of the cellulose/collagen nanofibrous conduit, its lumen was coated with either MSCs or pre-induced MSCs. The nerve conduit was then applied to the defective main trunk of the facial nerve. Rats were randomly divided into three treatment groups (n = 10 in each): cellulose/collagen nanofiber (control group), cellulose/collagen nanofiber/MSCs (group I), and cellulose/collagen nanofiber/pre-induced MSCs (group II). (3) Results Fibrillation of the vibrissae of each group was observed, and action potential threshold was compared 8 weeks post-surgery. Histopathological changes were also observed. Groups I and II showed better recovery of vibrissa fibrillation than the control group. (4) Conclusions: Group II, treated with the pre-induced MSC-coated cellulose/collagen nanofibrous nerve conduit, showed the highest degree of recovery based on functional and histological evaluations.

Citing Articles

Advancements in autologous peripheral nerve transplantation care: a review of strategies and practices to facilitate recovery.

Xu G, Zou X, Dong Y, Alhaskawi A, Zhou H, Hasan Abdullah Ezzi S Front Neurol. 2024; 15:1330224.

PMID: 38523615 PMC: 10959128. DOI: 10.3389/fneur.2024.1330224.

Porous Organic Materials in Tissue Engineering: Recent Advances and Applications for Severed Facial Nerve Injury Repair.

Sun J, Cao W, Pan S, He L, Ji D, Zheng N Molecules. 2024; 29(3).

PMID: 38338311 PMC: 10856494. DOI: 10.3390/molecules29030566.

Techniques and graft materials for repairing peripheral nerve defects.

Zou X, Dong Y, Alhaskawi A, Zhou H, Hasan Abdullah Ezzi S, Goutham Kota V Front Neurol. 2024; 14:1307883.

PMID: 38318237 PMC: 10839026. DOI: 10.3389/fneur.2023.1307883.

Effect of Metformin on the Functional and Electrophysiological Recovery of Crush Injury-Induced Facial Nerve Paralysis in Diabetic Rats.

Sun K, Choi C, Cho G, Jang C J Pers Med. 2023; 13(9).

PMID: 37763084 PMC: 10532940. DOI: 10.3390/jpm13091317.

Advances in Regenerative Dentistry Approaches: An Update.

Thalakiriyawa D, Dissanayaka W Int Dent J. 2023; 74(1):25-34.

PMID: 37541918 PMC: 10829373. DOI: 10.1016/j.identj.2023.07.008.

References

Jessen K, Mirsky R . The repair Schwann cell and its function in regenerating nerves. J Physiol. 2016; 594(13):3521-31. PMC: 4929314. DOI: 10.1113/JP270874. View

Urrutia D, Caviedes P, Mardones R, Minguell J, Vega-Letter A, Jofre C . Comparative study of the neural differentiation capacity of mesenchymal stromal cells from different tissue sources: An approach for their use in neural regeneration therapies. PLoS One. 2019; 14(3):e0213032. PMC: 6437714. DOI: 10.1371/journal.pone.0213032. View

Hu F, Wang X, Liang G, Lv L, Zhu Y, Sun B . Effects of epidermal growth factor and basic fibroblast growth factor on the proliferation and osteogenic and neural differentiation of adipose-derived stem cells. Cell Reprogram. 2013; 15(3):224-32. PMC: 3666248. DOI: 10.1089/cell.2012.0077. View

Qian Y, Zhou X, Zhang F, Diekwisch T, Luan X, Yang J . Triple PLGA/PCL Scaffold Modification Including Silver Impregnation, Collagen Coating, and Electrospinning Significantly Improve Biocompatibility, Antimicrobial, and Osteogenic Properties for Orofacial Tissue Regeneration. ACS Appl Mater Interfaces. 2019; 11(41):37381-37396. PMC: 7220812. DOI: 10.1021/acsami.9b07053. View

Balakrishnan A, Belfiore L, Chu T, Fleming T, Midha R, Biernaskie J . Insights Into the Role and Potential of Schwann Cells for Peripheral Nerve Repair From Studies of Development and Injury. Front Mol Neurosci. 2021; 13:608442. PMC: 7868393. DOI: 10.3389/fnmol.2020.608442. View

Joe I, Jeong S, Cho G . Resveratrol-induced SIRT1 activation promotes neuronal differentiation of human bone marrow mesenchymal stem cells. Neurosci Lett. 2014; 584:97-102. DOI: 10.1016/j.neulet.2014.10.024. View

Beris A, Gkiatas I, Gelalis I, Papadopoulos D, Kostas-Agnantis I . Current concepts in peripheral nerve surgery. Eur J Orthop Surg Traumatol. 2018; 29(2):263-269. DOI: 10.1007/s00590-018-2344-2. View

Scuteri A, Miloso M, Foudah D, Orciani M, Cavaletti G, Tredici G . Mesenchymal stem cells neuronal differentiation ability: a real perspective for nervous system repair?. Curr Stem Cell Res Ther. 2010; 6(2):82-92. DOI: 10.2174/157488811795495486. View

Farhang S, Soleimani M, Ostadsharif M, Ghasemi N . Neurogenic induction of human dental pulp derived stem cells by hanging drop technique, basic fibroblast growth factor, and SHH factors. Dent Res J (Isfahan). 2021; 18:57. PMC: 8404565. View

10.

Hussin H, Lawi M, Haflah N, Kassim A, Idrus R, Lokanathan Y . Centella asiatica (L.)-Neurodifferentiated Mesenchymal Stem Cells Promote the Regeneration of Peripheral Nerve. Tissue Eng Regen Med. 2020; 17(2):237-251. PMC: 7105542. DOI: 10.1007/s13770-019-00235-6. View

11.

Santos J, Milthorpe B, Herbert B, Padula M . Proteomic Analysis of Human Adipose Derived Stem Cells during Small Molecule Chemical Stimulated Pre-neuronal Differentiation. Int J Stem Cells. 2017; 10(2):193-217. PMC: 5741201. DOI: 10.15283/ijsc17036. View

12.

Rao F, Wang Y, Zhang D, Lu C, Cao Z, Sui J . Aligned chitosan nanofiber hydrogel grafted with peptides mimicking bioactive brain-derived neurotrophic factor and vascular endothelial growth factor repair long-distance sciatic nerve defects in rats. Theranostics. 2020; 10(4):1590-1603. PMC: 6993237. DOI: 10.7150/thno.36272. View

13.

Jessen K, Mirsky R, Lloyd A . Schwann Cells: Development and Role in Nerve Repair. Cold Spring Harb Perspect Biol. 2015; 7(7):a020487. PMC: 4484967. DOI: 10.1101/cshperspect.a020487. View

14.

Debski T, Kijenska-Gawronska E, Zolocinska A, Siennicka K, Slysz A, Paskal W . Bioactive Nanofiber-Based Conduits in a Peripheral Nerve Gap Management-An Animal Model Study. Int J Mol Sci. 2021; 22(11). PMC: 8197537. DOI: 10.3390/ijms22115588. View

15.

Alizadeh-Mohajer M, Raisi A, Farjanikish G, Mohammadi R . Effect of Local Administration of Verapamil Combined with Chitosan Based Hybrid Nanofiber Conduit on Transected Sciatic Nerve in Rat. Bull Emerg Trauma. 2019; 7(1):28-34. PMC: 6360005. DOI: 10.29252/beat-070104. View

16.

Hernandez R, Jimenez-Luna C, Perales-Adan J, Perazzoli G, Melguizo C, Prados J . Differentiation of Human Mesenchymal Stem Cells towards Neuronal Lineage: Clinical Trials in Nervous System Disorders. Biomol Ther (Seoul). 2019; 28(1):34-44. PMC: 6939692. DOI: 10.4062/biomolther.2019.065. View

17.

Frost H, Andersson T, Johansson S, Englund-Johansson U, Ekstrom P, Dahlin L . Electrospun nerve guide conduits have the potential to bridge peripheral nerve injuries in vivo. Sci Rep. 2018; 8(1):16716. PMC: 6233209. DOI: 10.1038/s41598-018-34699-8. View

18.

Matsuse D, Kitada M, Kohama M, Nishikawa K, Makinoshima H, Wakao S . Human umbilical cord-derived mesenchymal stromal cells differentiate into functional Schwann cells that sustain peripheral nerve regeneration. J Neuropathol Exp Neurol. 2010; 69(9):973-85. DOI: 10.1097/NEN.0b013e3181eff6dc. View

19.

Han Y, Li X, Zhang Y, Han Y, Chang F, Ding J . Mesenchymal Stem Cells for Regenerative Medicine. Cells. 2019; 8(8). PMC: 6721852. DOI: 10.3390/cells8080886. View

20.

Jang C, Lee S, Park I, Song A, Moon C, Cho G . Memantine Attenuates Salicylate-induced Tinnitus Possibly by Reducing NR2B Expression in Auditory Cortex of Rat. Exp Neurobiol. 2019; 28(4):495-503. PMC: 6751868. DOI: 10.5607/en.2019.28.4.495. View