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Complete Chloroplast Genome of an Endangered Species , and Its Comparative, Evolutionary, and Phylogenetic Study with Other Section Species

Overview
Journal Genes (Basel)
Publisher MDPI
Date 2022 Jul 27
PMID 35885967
Authors
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Abstract

, an endangered montane cloud forest species, is endemic to southern China. To understand the genomic features, phylogenetic relationships, and molecular evolution of , the complete chloroplast (cp) genome was analyzed and compared in section . The cp genome of was 160,782 bp in length, with an overall guanine and cytosine (GC) content of 36.9%. It contained 131 genes, including 86 protein-coding genes, eight ribosomal RNA genes, and 37 transfer RNA genes. A total of 165 simple sequence repeats (SSRs) and 48 long sequence repeats with A/T bias were identified in the cp genome, which were mainly distributed in the large single copy region (LSC) and intergenic spacer regions. The cp genome was similar in size, gene composition, and linearity of the structural region to those of species. The non-coding regions were more divergent than the coding regions, and the LSC region and small single copy region (SSC) were more divergent than the inverted repeat regions (IRs). Among the 13 divergent regions, 11 were in the LSC region, and only two were in the SSC region. Moreover, the coding sequence (CDS) of the six protein-coding genes (, , , , , and ) were subjected to positive selection pressure when pairwise comparison of 16 species of section . A close relationship between and was found in the phylogenetic analysis of cp genomes. Our study provided highly effective molecular markers for subsequent phylogenetic analysis, species identification, and biogeographic analysis of .

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