Kinetic Changes in B7 Costimulatory Molecules and IRF4 Expression in Human Dendritic Cells During LPS Exposure

Overview

Journal Biomolecules

Publisher MDPI

Specialties Biochemistry
Molecular Biology

Date 2022 Jul 27

PMID 35883511

Authors

Henry Velazquez-Soto

Fernanda Real-San Miguel

Sonia Mayra Perez-Tapia

Maria C Jimenez-Martinez

Affiliations

Soon will be listed here.

Abstract

A key aspect of the inflammatory phenomenon is the involvement of costimulatory molecules expressed by antigen-presenting cells (APCs) and their ability to secrete cytokines to set instructions for an adaptive immune response and to generate tolerance or inflammation. In a novel integrative approach, we aimed to evaluate the kinetic expression of the membrane and soluble B7 costimulatory molecules CD86, ICOS-L, PDL1, PDL2, the transcription factor Interferon Regulatory Factor 4 (IRF4), and the cytokines produced by monocyte-derived dendritic cells (Mo-DCs) after challenging them with different concentrations of stimulation with lipopolysaccharide (LPS) for different lengths of time. Our results showed that the stimuli concentration and time of exposure to an antigen are key factors in modulating the dynamic expression pattern of membrane and soluble B7 molecules and cytokines.

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References

Trevejo J, Marino M, Philpott N, Josien R, Richards E, Elkon K . TNF-alpha -dependent maturation of local dendritic cells is critical for activating the adaptive immune response to virus infection. Proc Natl Acad Sci U S A. 2001; 98(21):12162-7. PMC: 59785. DOI: 10.1073/pnas.211423598. View

Qian J, Wang C, Wang B, Yang J, Wang Y, Luo F . The IFN-γ/PD-L1 axis between T cells and tumor microenvironment: hints for glioma anti-PD-1/PD-L1 therapy. J Neuroinflammation. 2018; 15(1):290. PMC: 6192101. DOI: 10.1186/s12974-018-1330-2. View

Osugi Y, Vuckovic S, Hart D . Myeloid blood CD11c(+) dendritic cells and monocyte-derived dendritic cells differ in their ability to stimulate T lymphocytes. Blood. 2002; 100(8):2858-66. DOI: 10.1182/blood.V100.8.2858. View

Yi M, Niu M, Xu L, Luo S, Wu K . Regulation of PD-L1 expression in the tumor microenvironment. J Hematol Oncol. 2021; 14(1):10. PMC: 7792099. DOI: 10.1186/s13045-020-01027-5. View

Abdi K, Singh N, Matzinger P . Lipopolysaccharide-activated dendritic cells: "exhausted" or alert and waiting?. J Immunol. 2012; 188(12):5981-9. PMC: 3370068. DOI: 10.4049/jimmunol.1102868. View

Cabeza-Cabrerizo M, Cardoso A, Minutti C, Pereira da Costa M, Reis e Sousa C . Dendritic Cells Revisited. Annu Rev Immunol. 2021; 39:131-166. DOI: 10.1146/annurev-immunol-061020-053707. View

Skidmore B, Chiller J, Morrison D, Weigle W . Immunologic properties of bacterial lipopolysaccharide (LPS): correlation between the mitogenic, adjuvant, and immunogenic activities. J Immunol. 1975; 114(2 pt 2):770-5. View

Aicher A, Brady W, Pezzutto A, Richter G, Magaletti D, Buckwalter S . Characterization of human inducible costimulator ligand expression and function. J Immunol. 2000; 164(9):4689-96. DOI: 10.4049/jimmunol.164.9.4689. View

Gao Y, Nish S, Jiang R, Hou L, Licona-Limon P, Weinstein J . Control of T helper 2 responses by transcription factor IRF4-dependent dendritic cells. Immunity. 2013; 39(4):722-32. PMC: 4110745. DOI: 10.1016/j.immuni.2013.08.028. View

10.

Dustin M . The immunological synapse. Cancer Immunol Res. 2014; 2(11):1023-33. PMC: 4692051. DOI: 10.1158/2326-6066.CIR-14-0161. View

11.

Maurer D, Adamik J, Santos P, Shi J, Shurin M, Kirkwood J . Dysregulated NF-κB-Dependent ICOSL Expression in Human Dendritic Cell Vaccines Impairs T-cell Responses in Patients with Melanoma. Cancer Immunol Res. 2020; 8(12):1554-1567. PMC: 8018573. DOI: 10.1158/2326-6066.CIR-20-0274. View

12.

Rodriguez-Garcia M, Porichis F, de Jong O, Levi K, Diefenbach T, Lifson J . Expression of PD-L1 and PD-L2 on human macrophages is up-regulated by HIV-1 and differentially modulated by IL-10. J Leukoc Biol. 2010; 89(4):507-15. PMC: 3058820. DOI: 10.1189/jlb.0610327. View

13.

Hoebe K, Janssen E, Kim S, Alexopoulou L, Flavell R, Han J . Upregulation of costimulatory molecules induced by lipopolysaccharide and double-stranded RNA occurs by Trif-dependent and Trif-independent pathways. Nat Immunol. 2003; 4(12):1223-9. DOI: 10.1038/ni1010. View

14.

Montemagno C, Hagege A, Borchiellini D, Thamphya B, Rastoin O, Ambrosetti D . Soluble forms of PD-L1 and PD-1 as prognostic and predictive markers of sunitinib efficacy in patients with metastatic clear cell renal cell carcinoma. Oncoimmunology. 2020; 9(1):1846901. PMC: 7714499. DOI: 10.1080/2162402X.2020.1846901. View

15.

Peggs K, Allison J . Co-stimulatory pathways in lymphocyte regulation: the immunoglobulin superfamily. Br J Haematol. 2005; 130(6):809-24. DOI: 10.1111/j.1365-2141.2005.05627.x. View

16.

Xiong A, Wang J, Mao X, Jiang Y, Fan Y . MiR-199a-3p modulates the function of dendritic cells involved in transplantation tolerance by targeting CD86. HLA. 2019; 94(6):493-503. DOI: 10.1111/tan.13677. View

17.

Carenza C, Calcaterra F, Oriolo F, Di Vito C, Ubezio M, Della Porta M . Costimulatory Molecules and Immune Checkpoints Are Differentially Expressed on Different Subsets of Dendritic Cells. Front Immunol. 2019; 10:1325. PMC: 6579930. DOI: 10.3389/fimmu.2019.01325. View

18.

Yin X, Chen S, Eisenbarth S . Dendritic Cell Regulation of T Helper Cells. Annu Rev Immunol. 2021; 39:759-790. DOI: 10.1146/annurev-immunol-101819-025146. View

19.

Obendorf J, Viveros P, Fehlings M, Klotz C, Aebischer T, Ignatius R . Increased expression of CD25, CD83, and CD86, and secretion of IL-12, IL-23, and IL-10 by human dendritic cells incubated in the presence of Toll-like receptor 2 ligands and Giardia duodenalis. Parasit Vectors. 2014; 6(1):317. PMC: 4029533. DOI: 10.1186/1756-3305-6-317. View

20.

Her M, Kim D, Oh M, Jeong H, Choi I . Increased expression of soluble inducible costimulator ligand (ICOSL) in patients with systemic lupus erythematosus. Lupus. 2009; 18(6):501-7. DOI: 10.1177/0961203308099176. View