CXCR2 Is Essential for Radiation-Induced Intestinal Injury by Initiating Neutrophil Infiltration

Overview

Journal J Immunol Res

Publisher Wiley

Specialty Allergy & Immunology

Date 2022 Jul 26

PMID 35879949

Authors

Jing Hu

Qian Ji

Fei Chen

Xiaoqin Gong

Chuansheng Chen

Kaijun Zhang

Ye Hua

Jinlei Wang

Rongkun Li

Xu Wang

Chunhua Dai

Ye Tian

Affiliations

Soon will be listed here.

Abstract

Neutrophils, known as an important part of the immune system, are the most abundant leukocyte population in peripheral blood, but excessive recruitment will lead to tissue/organ injury. RNA sequencing showed that ionizing radiation significantly increased the expression of characteristic genes of neutrophils in intestinal tissues compared with liver and lung tissues. By clearing neutrophils with an anti-Ly6G antibody, we found that neutrophil infiltration is critical for irradiation-induced intestinal injury. CXCR2 is a G-protein-coupled receptor that mediates the migration of neutrophils by combining with its ligands. Compared with observations in liver and lung tissues, we found that CXCR2 and its ligands, including CXCL1, CXCL2, CXCL3, and CXCL5, were all significantly upregulated in irradiated intestinal tissues. Further studies showed that SB225002, an inhibitor of CXCR2, could effectively inhibit the chemotaxis of neutrophils and tissue damage mediated by the CXCL-CXCR2 signalling pathway.

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