Comparison Between Radiolabelled and Endogenous Dopamine Release from Rat Striatal Slices: Effects of Electrical Field Stimulation and Regulation by D2-autoreceptors

Overview

Journal Naunyn Schmiedebergs Arch Pharmacol

Specialty Pharmacology

Date 1987 Mar 1

PMID 3587370

Citations 7

Authors

H Herdon

S R Nahorski

Affiliations

Soon will be listed here.

Abstract

Direct comparisons have been made between the release of radiolabelled and endogenous dopamine from superfused rat striatal slices prelabelled with 3H-dopamine. Both spontaneous release and release evoked by electrical field stimulation (3 Hz, 2 min) were measured using a high-sensitivity HPLC system with electrochemical (coulometric) detection, plus scintillation counting of chromatographically separated superfusate fractions. Two periods of electrical stimulation released similar amounts of endogenous dopamine, but the second stimulation released much less 3H-dopamine than did the first, although the levels of spontaneous release immediately before the two stimuli were similar. Substantial increases in endogenous 3,4-dihydroxy phenyl acetic acid (DOPAC) release but only minor increases in 3H-DOPAC release occurred following the two stimuli. The dopamine agonist pergolide (1 microM) reduced the electrically-stimulated release of both 3H-dopamine and endogenous dopamine to a similar extent, whilst the D2-selective antagonist sulpiride (1 microM) produced large increases in both 3H-dopamine and endogenous dopamine electrically-stimulated release. In addition, spontaneous release of both 3H-dopamine and endogenous dopamine were decreased by pergolide and increased by sulpiride. Co-addition of sulpiride and pergolide produced lesser increases than those seen with sulpiride alone. These studies indicate that, despite major differences between 3H-dopamine and endogenous dopamine release in response to various stimuli, their regulation by D2-autoreceptors appears similar; a novel finding being the modulation of spontaneous 3H-dopamine release by autoreceptors.(ABSTRACT TRUNCATED AT 250 WORDS)

Citing Articles

Dopaminergic impact of cART and anti-depressants on HIV neuropathogenesis in older adults.

Matt S, Gaskill P Brain Res. 2019; 1723:146398.

PMID: 31442412 PMC: 6766423. DOI: 10.1016/j.brainres.2019.146398.

Reduced inhibitory action of a GABAB receptor agonist on [3H]-dopamine release from rat ventral tegmental area in vitro after chronic nicotine administration.

Amantea D, Bowery N BMC Pharmacol. 2004; 4:24.

PMID: 15494079 PMC: 526276. DOI: 10.1186/1471-2210-4-24.

Electrically induced release of endogenous noradrenaline and dopamine from brain slices: pseudo-one-pulse stimulation utilized to study presynaptic autoinhibition.

Thienprasert A, Singer E Naunyn Schmiedebergs Arch Pharmacol. 1993; 348(2):119-26.

PMID: 8232591 DOI: 10.1007/BF00164787.

Proceedings of the British Pharmacological Society. Liverpool, 6th-8th April, 1988. Abstracts.

Br J Pharmacol. 1988; 94 Suppl:312P-474P.

PMID: 3382861 PMC: 1853740.

Neurotensin effects on evoked release of dopamine in slices from striatum, nucleus accumbens and prefrontal cortex in rat.

Hetier E, Boireau A, Dubedat P, Blanchard J Naunyn Schmiedebergs Arch Pharmacol. 1988; 337(1):13-7.

PMID: 3368012 DOI: 10.1007/BF00169470.

References

Starke K, Spath L, Lang J, Adelung C . Further functional in vitro comparison of pre- and postsynaptic dopamine receptors in the rabbit caudate nucleus. Naunyn Schmiedebergs Arch Pharmacol. 1983; 323(4):298-306. DOI: 10.1007/BF00512467. View

Parker E, Cubeddu L . Evidence for autoreceptor modulation of endogenous dopamine release from rabbit caudate nucleus in vitro. J Pharmacol Exp Ther. 1985; 232(2):492-500. View

Herdon H, Strupish J, Nahorski S . Differences between the release of radiolabelled and endogenous dopamine from superfused rat brain slices: effects of depolarizing stimuli, amphetamine and synthesis inhibition. Brain Res. 1985; 348(2):309-20. DOI: 10.1016/0006-8993(85)90450-0. View

Heikkila R, Manzino L . Behavioral properties of GBR 12909, GBR 13069 and GBR 13098: specific inhibitors of dopamine uptake. Eur J Pharmacol. 1984; 103(3-4):241-8. DOI: 10.1016/0014-2999(84)90483-7. View

Zumstein A, Karduck W, Starke K . Pathways of dopamine metabolism in the rabbit caudate nucleus in vitro. Naunyn Schmiedebergs Arch Pharmacol. 1981; 316(3):205-17. DOI: 10.1007/BF00505651. View

Lehmann J, Briley M, Langer S . Characterization of dopamine autoreceptor and [3H]spiperone binding sites in vitro with classical and novel dopamine receptor agonists. Eur J Pharmacol. 1983; 88(1):11-26. DOI: 10.1016/0014-2999(83)90387-4. View

Herdon H, Strupish J, Nahorski S . Endogenous dopamine release from rat striatal slices and its regulation by D-2 autoreceptors: effects of uptake inhibitors and synthesis inhibition. Eur J Pharmacol. 1987; 138(1):69-76. DOI: 10.1016/0014-2999(87)90338-4. View

Markstein R, Lahaye D . In vitro effect of the racemic mixture and the (-)enantiomer of N-n-propyl-3-(3-hydroxyphenyl)-piperidine (3-PPP) on postsynaptic dopamine receptors and on a presynaptic dopamine autoreceptor. J Neural Transm. 1983; 58(1-2):43-53. DOI: 10.1007/BF01249123. View

Niddam R, Arbilla S, Scatton B, Dennis T, Langer S . Amphetamine induced release of endogenous dopamine in vitro is not reduced following pretreatment with reserpine. Naunyn Schmiedebergs Arch Pharmacol. 1985; 329(2):123-7. DOI: 10.1007/BF00501200. View

10.

Plotsky P, Wightman R, Chey W, Adams R . Liquid chromatographic analysis of endogenous catecholamine release from brain slices. Science. 1977; 197(4306):904-6. DOI: 10.1126/science.887928. View

11.

Starke K . Presynaptic receptors. Annu Rev Pharmacol Toxicol. 1981; 21:7-30. DOI: 10.1146/annurev.pa.21.040181.000255. View

12.

Trendelenburg U, STEFANO F, Grohmann M . The isotope effect of tritium in 3H-noradrenaline. Naunyn Schmiedebergs Arch Pharmacol. 1983; 323(2):128-40. DOI: 10.1007/BF00634260. View

13.

McMILLEN B, German D, Shore P . Functional and pharmacological significance of brain dopamine and norepinephrine storage pools. Biochem Pharmacol. 1980; 29(22):3045-50. DOI: 10.1016/0006-2952(80)90444-x. View

14.

Grohmann M, Henseling M, Cassis L, Trendelenburg U . Errors introduced by a tritium label in position 8 of catecholamines. Naunyn Schmiedebergs Arch Pharmacol. 1986; 332(1):34-42. DOI: 10.1007/BF00633194. View

15.

Cubeddu L, Hoffmann I, Ferrari G . Metabolism and efflux of [3H]dopamine in rat neostriatum: presynaptic origin of 3,4-[3H]dihydroxyphenylacetic acid. J Pharmacol Exp Ther. 1979; 209(2):165-75. View

16.

Markstein R, Hokfelt T . Effect of cholecystokinin-octapeptide on dopamine release from slices of cat caudate nucleus. J Neurosci. 1984; 4(2):570-5. PMC: 6564911. View

17.

Langer S . Presynaptic regulation of the release of catecholamines. Pharmacol Rev. 1980; 32(4):337-62. View

18.

Milner J, Wurtman R . Release of endogenous dopamine from electrically stimulated slices of rat striatum. Brain Res. 1984; 301(1):139-42. DOI: 10.1016/0006-8993(84)90410-4. View

19.

Bonnet J, Costentin J . GBR 12783, a potent and selective inhibitor of dopamine uptake: biochemical studies in vivo and ex vivo. Eur J Pharmacol. 1986; 121(2):199-209. DOI: 10.1016/0014-2999(86)90491-7. View

20.

Parker E, Cubeddu L . Effects of d-amphetamine and dopamine synthesis inhibitors on dopamine and acetylcholine neurotransmission in the striatum. II. Release in the presence of vesicular transmitter stores. J Pharmacol Exp Ther. 1986; 237(1):193-203. View