Methanol Biotransformation Toward High-level Production of Fatty Acid Derivatives by Engineering the Industrial Yeast

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 2022 Jul 20

PMID 35858340

Authors

Peng Cai

Xiaoyan Wu

Jun Deng

Linhui Gao

Yiwei Shen

Lun Yao

Yongjin J Zhou

Affiliations

Soon will be listed here.

Abstract

Methanol-based biorefinery is a promising strategy to achieve carbon neutrality goals by linking CO capture and solar energy storage. As a typical methylotroph, shows great potential in methanol biotransformation. However, challenges still remain in engineering methanol metabolism for chemical overproduction. Here, we present the global rewiring of the central metabolism for efficient production of free fatty acids (FFAs; 23.4 g/L) from methanol, with an enhanced supply of precursors and cofactors, as well as decreased accumulation of formaldehyde. Finally, metabolic transforming of the fatty acid cell factory enabled overproduction of fatty alcohols (2.0 g/L) from methanol. This study demonstrated that global metabolic rewiring released the great potential of for methanol biotransformation toward chemical overproduction.

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