Wenshen Yangxue Decoction Promotes Follicular Development in Aged Female Mice Stimulation of the Silent Information Regulator 3/forkhead Transcription Factor O1 3a Pathway

Overview

Journal J Tradit Chin Med

Date 2022 Jul 18

PMID 35848970

Authors

Zhang Liang

Han Qian

Y I Yanxiao

J I Shaoyang

Xin Mingwei

Affiliations

Soon will be listed here.

Abstract

Objective: To primarily explore the effect and mechanism of Wenshen Yangxue decoction in promoting follicular development in elderly female mice.

Methods: Fifty Institute of Cancer Research mice were randomly divided into blank, controlled ovarian hyperstimulation (COH), low-dose Wenshen Yangxue decoction, medium-dose Wenshen Yangxue decoction, and high-dose Wenshen Yangxue decoction groups, with 10 mice in each group. The number of ovulations, number of fertilizations, mitochondrial adenosine triphosphate (ATP) level, and mitochondrial DNA (mtDNA) of oocytes in each group were compared. Reverse transcription-polymerase chain reaction and Western blotting were used to detect the mRNA and protein expression levels of silent information regulator 3 (Sirt3) and forkhead transcription factor O1 3a (FOXO3a).

Results: Wenshen Yangxue decoction significantly increased the number of ovulations in mice (P < 0.05) and promoted the formation of fertilized eggs. The ATP level and mtDNA copy number of mice oocytes in the high-dose groups were significantly higher than those in the COH group (P < 0.05). Wenshen Yangxue decoction significantly increased the mRNA and protein levels of Sirt3 and FOXO3a in mouse oocytes.

Conclusion: Wenshen Yangxue decoction promoted the development of follicles in elderly female mice, increased the number of ovulations and improved fertility. Its mechanism may be related to increased mitochondrial energy metabolism and regulation of the Sirt3/FOXO3a pathway.

References

Pasquariello R, Ermisch A, Silva E, McCormick S, Logsdon D, Barfield J . Alterations in oocyte mitochondrial number and function are related to spindle defects and occur with maternal aging in mice and humans†. Biol Reprod. 2018; 100(4):971-981. DOI: 10.1093/biolre/ioy248. View

Mobarak H, Heidarpour M, Tsai P, Rezabakhsh A, Rahbarghazi R, Nouri M . Autologous mitochondrial microinjection; a strategy to improve the oocyte quality and subsequent reproductive outcome during aging. Cell Biosci. 2019; 9:95. PMC: 6884882. DOI: 10.1186/s13578-019-0360-5. View

Xin M, He J, Yang W, Yin X, Wang J . Wenshen Yangxue decoction improves endometrial receptivity recovery and promotes endometrial angiogenesis in a rat model. Pharm Biol. 2019; 56(1):573-579. PMC: 6292361. DOI: 10.1080/13880209.2018.1510973. View

Lliberos C, Liew S, Zareie P, La Gruta N, Mansell A, Hutt K . Evaluation of inflammation and follicle depletion during ovarian ageing in mice. Sci Rep. 2021; 11(1):278. PMC: 7801638. DOI: 10.1038/s41598-020-79488-4. View

Zhao H, Ding T, Ren Y, Li T, Li R, Fan Y . Role of Sirt3 in mitochondrial biogenesis and developmental competence of human in vitro matured oocytes. Hum Reprod. 2016; 31(3):607-22. DOI: 10.1093/humrep/dev345. View

Iljas J, Homer H . Sirt3 is dispensable for oocyte quality and female fertility in lean and obese mice. FASEB J. 2020; 34(5):6641-6653. DOI: 10.1096/fj.202000153R. View

Patel R, Moffatt J, Mourmoura E, Demaison L, Seed P, Poston L . Effect of reproductive ageing on pregnant mouse uterus and cervix. J Physiol. 2017; 595(6):2065-2084. PMC: 5350451. DOI: 10.1113/JP273350. View

Ruankham W, Suwanjang W, Wongchitrat P, Prachayasittikul V, Prachayasittikul S, Phopin K . Sesamin and sesamol attenuate HO-induced oxidative stress on human neuronal cells via the SIRT1-SIRT3-FOXO3a signaling pathway. Nutr Neurosci. 2019; 24(2):90-101. DOI: 10.1080/1028415X.2019.1596613. View

Zhang T, Xi Q, Wang D, Li J, Wang M, Li D . Mitochondrial dysfunction and endoplasmic reticulum stress involved in oocyte aging: an analysis using single-cell RNA-sequencing of mouse oocytes. J Ovarian Res. 2019; 12(1):53. PMC: 6556043. DOI: 10.1186/s13048-019-0529-x. View

10.

Xin M, He J, Zhang Y, Wu Y, Yang W, Liang X . Chinese herbal decoction of Wenshen Yangxue formula improved fertility and pregnancy rate in mice through PI3K/Akt signaling. J Cell Biochem. 2018; 120(3):3082-3090. DOI: 10.1002/jcb.27483. View

11.

Katz-Jaffe M, Lane S, Parks J, McCallie B, Makloski R, Schoolcraft W . Antioxidant Intervention Attenuates Aging-Related Changes in the Murine Ovary and Oocyte. Life (Basel). 2020; 10(11). PMC: 7690403. DOI: 10.3390/life10110250. View

12.

Maidarti M, Anderson R, Telfer E . Crosstalk between PTEN/PI3K/Akt Signalling and DNA Damage in the Oocyte: Implications for Primordial Follicle Activation, Oocyte Quality and Ageing. Cells. 2020; 9(1). PMC: 7016612. DOI: 10.3390/cells9010200. View

13.

Liu J, Wang Y, Hao Y, Wang Z, Yang Z, Wang Z . 5-Heptadecylresorcinol attenuates oxidative damage and mitochondria-mediated apoptosis through activation of the SIRT3/FOXO3a signaling pathway in neurocytes. Food Funct. 2020; 11(3):2535-2542. DOI: 10.1039/c9fo03028j. View

14.

Fan L, Wang Z, Li Q, Meng T, Dong M, Hou Y . Absence of mitochondrial DNA methylation in mouse oocyte maturation, aging and early embryo development. Biochem Biophys Res Commun. 2019; 513(4):912-918. DOI: 10.1016/j.bbrc.2019.04.100. View

15.

Busnelli A, Navarra A, Levi-Setti P . Qualitative and Quantitative Ovarian and Peripheral Blood Mitochondrial DNA (mtDNA) Alterations: Mechanisms and Implications for Female Fertility. Antioxidants (Basel). 2021; 10(1). PMC: 7824846. DOI: 10.3390/antiox10010055. View

16.

Liu M, Chen H, Li W, Chen X, Wang C, Sun Q . SRT1720 enhances maturity and quality of oocytes in aged mice. Anim Sci J. 2021; 92(1):e13608. DOI: 10.1111/asj.13608. View

17.

Wang S, Yang J, Lin T, Huang S, Ma J, Xu X . Excessive production of mitochondrion‑derived reactive oxygen species induced by titanium ions leads to autophagic cell death of osteoblasts via the SIRT3/SOD2 pathway. Mol Med Rep. 2020; 22(1):257-264. PMC: 7248520. DOI: 10.3892/mmr.2020.11094. View

18.

Shi Y, He R, Yang Y, He Y, Zhan L, Wei B . Potential relationship between Sirt3 and autophagy in ovarian cancer. Oncol Lett. 2020; 20(5):162. PMC: 7471650. DOI: 10.3892/ol.2020.12023. View

19.

Ackert-Bicknell C, Anderson L, Sheehan S, Hill W, Chang B, Churchill G . Aging Research Using Mouse Models. Curr Protoc Mouse Biol. 2015; 5(2):95-133. PMC: 4590775. DOI: 10.1002/9780470942390.mo140195. View

20.

Zhou J, Xue Z, He H, Liu X, Yin S, Wu D . Resveratrol delays postovulatory aging of mouse oocytes through activating mitophagy. Aging (Albany NY). 2019; 11(23):11504-11519. PMC: 6932885. DOI: 10.18632/aging.102551. View