MiR-199a/b-3p Inhibits Colorectal Cancer Cell Proliferation, Migration and Invasion Through Targeting PAK4 and BCAR3

Overview

Journal Eur J Med Res

Publisher Biomed Central

Specialty General Medicine

Date 2022 Jul 16

PMID 35842733

Authors

Junjie Hou

Xuguang Mi

Ning Liu

Xiaonan Li

Xiao-Nan Li

Ying Yang

Xiaodan Lu

Yanqiu Fang

Ning-Yi Jin

Affiliations

Soon will be listed here.

Abstract

Background: Colorectal cancer (CRC) is one of the leading causes of cancer-related death worldwide. P21 activated kinase 4 (PAK4) and Breast cancer anti-estrogen resistance 3 (BCAR3) have been reported to be involved in numerous aspects in tumorous progression. In this study, we propose to screen multi-targeted microRNAs. (miRNAs), which simultaneously inhibit neoplastic evolution through suppressing the transcription of target genes.

Methods: MTT and Colony formation assays measured cell's viability and proliferation. Scratch wound and Transwell assays detected the ability in migration and invasion for SW116 cells. The multi-targeted microRNAs of PAK4 and BCAR3 were predicted using bioinformatics analysis and verified by conducting dual luciferase reporter assay, western blot and qRT-PCR that could detect the expression levels of miR-199a/b-3p.

Results: The knockdown of PAK4 significantly impeded proliferation and colony formation of SW1116 cells when the knockdown of BCAR3 hindered migration and invasion of SW1116 cells. MiR-199a/b-3p directly targeted the 3'-UTR of PAK4 and BCAR3, further effected proliferation, colony formation, migration, and invasion of SW1116 cells. PAK4 or BCAR3 overexpression could partially reversed inhibitory effects of miR-199a/b-3p.

Conclusions: These results provided a new multi-targeted cite for cancerous suppressant to improve the prognosis of CRC inpatients.

Citing Articles

Shi H, Huang S, Ma X, Tan Z, Luo R, Luo B World J Gastrointest Oncol. 2024; 16(7):3082-3096.

PMID: 39072167 PMC: 11271796. DOI: 10.4251/wjgo.v16.i7.3082.

Deciphering the Role of BCAR3 in Cancer Progression: Gene Regulation, Signal Transduction, and Therapeutic Implications.

Moon D Cancers (Basel). 2024; 16(9).

PMID: 38730626 PMC: 11083344. DOI: 10.3390/cancers16091674.

miR-199a/b-3p inhibits HCC cell proliferation and invasion through a novel compensatory signaling pathway DJ-1\Ras\PI3K/AKT.

Ma L, Wu L, Liu S, Zhang X, Luo X, Nawaz S Sci Rep. 2024; 14(1):224.

PMID: 38168113 PMC: 10762019. DOI: 10.1038/s41598-023-48760-8.

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