Applications of Polymerase Chain Reaction-based Methods for the Diagnosis of Plague (Review)

Overview

Journal Exp Ther Med

Specialty Pathology

Date 2022 Jul 15

PMID 35837060

Authors

Yanan Zhang

Zhanli Wang

Wenrui Wang

Hui Yu

Min Jin

Affiliations

Soon will be listed here.

Abstract

Plague is an acute bacterial infection caused by . The three major clinical forms of plague are bubonic, pneumonic and septicemic, which have high case-mortality rates. Therefore, rapid and reliable diagnostic tools are crucial. Currently, bacteriological means and traditional serological assays are used for detecting infection with . However, such methods have their limitations. Polymerase chain reaction (PCR) is one of the most useful tools for rapid diagnosis of plague. The present review introduced the main PCR techniques and their applications for detecting and confirmation of . The advantages and disadvantages of the different PCR methods were also summarized.

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