Integrated Single-cell Analyses Decode the Developmental Landscape of the Human Fetal Spine

Overview

Journal iScience

Publisher Cell Press

Date 2022 Jul 14

PMID 35832888

Authors

Haiyan Yu

Donge Tang

Hongwei Wu

Chunhong Li

Yongping Lu

Fang He

Xiaogang Zhang

Yane Yang

Wei Shi

Wenlong Hu

Zhipeng Zeng

Weier Dai

Minglin Ou

Yong Dai

Affiliations

Soon will be listed here.

Abstract

The spine has essential roles in supporting body weight, and passaging the neural elements between the body and the brain. In this study, we used integrated single-cell RNA sequencing and single-cell transposase-accessible chromatin sequencing analyses to reveal the cellular heterogeneity, lineage, and transcriptional regulatory network of the developing human spine. We found that + + fibroblasts with stem cell characteristics could differentiate into chondrocytes by highly expressing the chondrogenic markers and . Neurons could originate from neuroendocrine cells, and MEIS2 may be an essential transcription factor that promotes spinal neural progenitor cells to selectively differentiate into neurons during early gestation. Furthermore, the interaction of NRP2_SEMA3C and CD74_APP between macrophages and neurons may be essential for spinal cord development. Our integrated map provides a blueprint for understanding human spine development in the early and midgestational stages at single-cell resolution and offers a tool for investigating related diseases.

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