Generation of Genome-edited Dogs by Somatic Cell Nuclear Transfer

Overview

Journal BMC Biotechnol

Publisher Biomed Central

Specialty Biotechnology

Date 2022 Jul 13

PMID 35831828

Authors

Dong-Ern Kim

Ji-Hye Lee

Kuk-Bin Ji

Kang-Sun Park

Tae-Young Kil

Okjae Koo

Min-Kyu Kim

Affiliations

Soon will be listed here.

Abstract

Background: Canine cloning technology based on somatic cell nuclear transfer (SCNT) combined with genome-editing tools such as CRISPR-Cas9 can be used to correct pathogenic mutations in purebred dogs or to generate animal models of disease.

Results: We constructed a CRISPR-Cas9 vector targeting canine DJ-1. Genome-edited canine fibroblasts were established using vector transfection and antibiotic selection. We performed canine SCNT using genome-edited fibroblasts and successfully generated two genome-edited dogs. Both genome-edited dogs had insertion-deletion mutations at the target locus, and DJ-1 expression was either downregulated or completely repressed.

Conclusion: SCNT successfully produced genome-edited dogs by using the CRISPR-Cas9 system for the first time.

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