Integrated Transcriptomic and Regulatory Network Analyses Uncovers the Role of Let-7b-5p, SPIB, and HLA-DPB1 in Sepsis

Overview

Journal Sci Rep

Specialty Science

Date 2022 Jul 13

PMID 35831411

Authors

Mohd Mohsin

Prithvi Singh

Salman Khan

Amit Kumar Verma

Rishabh Jha

Mohammed A Alsahli

Arshad Husain Rahmani

Saleh A Almatroodi

Faris Alrumaihi

Nisha Kaprwan

Kapil Dev

Ravins Dohare

Mansoor Ali Syed

Affiliations

Soon will be listed here.

Abstract

Sepsis has affected millions of populations of all age groups, locations, and sexes worldwide. Immune systems, either innate or adaptive are dysregulated due to the infection. Various biomarkers are present to date, still sepsis is a primary cause of mortality. Globally, post-operative body infections can cause sepsis and septic shock in ICU. Abnormal antigen presentation to T-cells leads to a dysregulated immune system. miRNAs are sparkly evolved as biomarkers due to their high sensitivity and efficiency. In this work, we analyzed high-throughput mRNA data collected from Gene Expression Omnibus (GEO) and linked it to significant miRNAs and TFs using a network-based approach. Protein-protein interaction (PPI) network was constructed using sepsis-specific differentially expressed genes (DEGs) followed by enrichment analyses and hub module detection. Sepsis-linked decrease transcription of the classical HLA gene such as HLA-DPB1 and its interplay with miR-let-7b-5p and transcription factor SPIB was observed. This study helped to provide innovative targets for sepsis.

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