Factors Influencing Substrate Oxidation During Submaximal Cycling: A Modelling Analysis

Overview

Journal Sports Med

Specialty Orthopedics

Date 2022 Jul 13

PMID 35829994

Authors

Jeffrey A Rothschild

Andrew E Kilding

Tom Stewart

Daniel J Plews

Affiliations

Soon will be listed here.

Abstract

Background: Multiple factors influence substrate oxidation during exercise including exercise duration and intensity, sex, and dietary intake before and during exercise. However, the relative influence and interaction between these factors is unclear.

Objectives: Our aim was to investigate factors influencing the respiratory exchange ratio (RER) during continuous exercise and formulate multivariable regression models to determine which factors best explain RER during exercise, as well as their relative influence.

Methods: Data were extracted from 434 studies reporting RER during continuous cycling exercise. General linear mixed-effect models were used to determine relationships between RER and factors purported to influence RER (e.g., exercise duration and intensity, muscle glycogen, dietary intake, age, and sex), and to examine which factors influenced RER, with standardized coefficients used to assess their relative influence.

Results: The RER decreases with exercise duration, dietary fat intake, age, VO, and percentage of type I muscle fibers, and increases with dietary carbohydrate intake, exercise intensity, male sex, and carbohydrate intake before and during exercise. The modelling could explain up to 59% of the variation in RER, and a model using exclusively easily modified factors (exercise duration and intensity, and dietary intake before and during exercise) could only explain 36% of the variation in RER. Variables with the largest effect on RER were sex, dietary intake, and exercise duration. Among the diet-related factors, daily fat and carbohydrate intake have a larger influence than carbohydrate ingestion during exercise.

Conclusion: Variability in RER during exercise cannot be fully accounted for by models incorporating a range of participant, diet, exercise, and physiological characteristics. To better understand what influences substrate oxidation during exercise further research is required on older subjects and females, and on other factors that could explain additional variability in RER.

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References

Baldwin J, Snow R, Febbraio M . Effect of training status and relative exercise intensity on physiological responses in men. Med Sci Sports Exerc. 2000; 32(9):1648-54. DOI: 10.1097/00005768-200009000-00020. View

Mittendorfer B, Horowitz J, Klein S . Effect of gender on lipid kinetics during endurance exercise of moderate intensity in untrained subjects. Am J Physiol Endocrinol Metab. 2002; 283(1):E58-65. DOI: 10.1152/ajpendo.00504.2001. View

Leckey J, Hoffman N, Parr E, Devlin B, Trewin A, Stepto N . High dietary fat intake increases fat oxidation and reduces skeletal muscle mitochondrial respiration in trained humans. FASEB J. 2018; 32(6):2979-2991. DOI: 10.1096/fj.201700993R. View

OHara J, Woods D, Mellor A, Boos C, Gallagher L, Tsakirides C . A comparison of substrate oxidation during prolonged exercise in men at terrestrial altitude and normobaric normoxia following the coingestion of 13C glucose and 13C fructose. Physiol Rep. 2017; 5(1). PMC: 5256160. DOI: 10.14814/phy2.13101. View

Terink R, Witkamp R, Hopman M, Siebelink E, Savelkoul H, Mensink M . A 2 Week Cross-over Intervention with a Low Carbohydrate, High Fat Diet Compared to a High Carbohydrate Diet Attenuates Exercise-Induced Cortisol Response, but Not the Reduction of Exercise Capacity, in Recreational Athletes. Nutrients. 2021; 13(1). PMC: 7825040. DOI: 10.3390/nu13010157. View

Helge J, Fraser A, Kriketos A, Jenkins A, Calvert G, Ayre K . Interrelationships between muscle fibre type, substrate oxidation and body fat. Int J Obes Relat Metab Disord. 1999; 23(9):986-91. DOI: 10.1038/sj.ijo.0801030. View

Jamurtas A, Tofas T, Fatouros I, Nikolaidis M, Paschalis V, Yfanti C . The effects of low and high glycemic index foods on exercise performance and beta-endorphin responses. J Int Soc Sports Nutr. 2011; 8:15. PMC: 3219660. DOI: 10.1186/1550-2783-8-15. View

Lundsgaard A, Fritzen A, Kiens B . Molecular Regulation of Fatty Acid Oxidation in Skeletal Muscle during Aerobic Exercise. Trends Endocrinol Metab. 2017; 29(1):18-30. DOI: 10.1016/j.tem.2017.10.011. View

Parolin M, Wong D, Jones N, Heigenhauser G . Effect of induced metabolic acidosis on human skeletal muscle metabolism during exercise. Am J Physiol. 1999; 277(4):E647-58. DOI: 10.1152/ajpendo.1999.277.4.E647. View

10.

Bergman B, Brooks G . Respiratory gas-exchange ratios during graded exercise in fed and fasted trained and untrained men. J Appl Physiol (1985). 1999; 86(2):479-87. DOI: 10.1152/jappl.1999.86.2.479. View

11.

Suh S, Casazza G, Horning M, Miller B, Brooks G . Effects of oral contraceptives on glucose flux and substrate oxidation rates during rest and exercise. J Appl Physiol (1985). 2002; 94(1):285-94. DOI: 10.1152/japplphysiol.00693.2002. View

12.

Andersson Hall U, Edin F, Pedersen A, Madsen K . Whole-body fat oxidation increases more by prior exercise than overnight fasting in elite endurance athletes. Appl Physiol Nutr Metab. 2016; 41(4):430-7. DOI: 10.1139/apnm-2015-0452. View

13.

Chrzanowski-Smith O, Edinburgh R, Thomas M, Hengist A, Williams S, Betts J . Determinants of Peak Fat Oxidation Rates During Cycling in Healthy Men and Women. Int J Sport Nutr Exerc Metab. 2021; 31(3):227-235. DOI: 10.1123/ijsnem.2020-0262. View

14.

Virk R, Dunton N, Young J, Leklem J . Effect of vitamin B-6 supplementation on fuels, catecholamines, and amino acids during exercise in men. Med Sci Sports Exerc. 1999; 31(3):400-8. DOI: 10.1097/00005768-199903000-00008. View

15.

Coyle E, Coggan A, Hemmert M, Ivy J . Muscle glycogen utilization during prolonged strenuous exercise when fed carbohydrate. J Appl Physiol (1985). 1986; 61(1):165-72. DOI: 10.1152/jappl.1986.61.1.165. View

16.

King A, OHara J, Arjomandkhah N, Rowe J, Morrison D, Preston T . Liver and muscle glycogen oxidation and performance with dose variation of glucose-fructose ingestion during prolonged (3 h) exercise. Eur J Appl Physiol. 2019; 119(5):1157-1169. PMC: 6469629. DOI: 10.1007/s00421-019-04106-9. View

17.

Kuo J, Wen-Chyuan Chen K, Cheng I, Tsai P, Lu Y, Lee N . The effect of eight weeks of supplementation with Eleutherococcus senticosus on endurance capacity and metabolism in human. Chin J Physiol. 2011; 53(2):105-11. DOI: 10.4077/cjp.2010.amk018. View

18.

Jeukendrup A, Saris W, Schrauwen P, Brouns F, Wagenmakers A . Metabolic availability of medium-chain triglycerides coingested with carbohydrates during prolonged exercise. J Appl Physiol (1985). 1995; 79(3):756-62. DOI: 10.1152/jappl.1995.79.3.756. View

19.

Tarnopolsky L, MacDougall J, Atkinson S, Tarnopolsky M, Sutton J . Gender differences in substrate for endurance exercise. J Appl Physiol (1985). 1990; 68(1):302-8. DOI: 10.1152/jappl.1990.68.1.302. View

20.

Rothschild J, Kilding A, Plews D . What Should I Eat before Exercise? Pre-Exercise Nutrition and the Response to Endurance Exercise: Current Prospective and Future Directions. Nutrients. 2020; 12(11). PMC: 7696145. DOI: 10.3390/nu12113473. View