Class I Polyhydroxyalkanoate (PHA) Synthase Increased Polylactic Acid Production in Engineered Escherichia Coli

Overview

Journal Front Bioeng Biotechnol

Date 2022 Jul 11

PMID 35814019

Authors

Mengxun Shi

Mengdi Li

Anran Yang

Xue Miao

Liu Yang

Jagroop Pandhal

Huibin Zou

Affiliations

Soon will be listed here.

Abstract

Polylactic acid (PLA), a homopolymer of lactic acid (LA), is a bio-derived, biocompatible, and biodegradable polyester. The evolved class II PHA synthase (PhaC1 ) was commonly utilized in the biosynthesis of PLA from biomass. This study tested alternative class I PHA synthase (PhaC ) from sp. USM2 in engineered for the biosynthesis of PLA from glucose. The results indicated that PhaC had better performance in PLA production than that of class II synthase PhaC1 . In addition, the gene was engineered in PLA-producing strains for morphological engineering. The morphologically engineered strains present increased PLA production. This study also tested fused propionyl-CoA transferase and lactate dehydrogenase A (fused Pct /LdhA) in engineered and found that fused Pct /LdhA did not apparently improve the PLA production. After systematic engineering, the highest PLA production was achieved by MS6 (with and ), which could produce up to 955.0 mg/L of PLA in fed-batch fermentation with the cell dry weights of 2.23%, and the average molecular weight of produced PLA could reach 21,000 Da.

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