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Application of a Simple Methylation Procedure for the Analyses of Glycosphingolipids

Overview
Journal Carbohydr Res
Publisher Elsevier
Date 1987 Apr 1
PMID 3581110
Citations 20
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Abstract

Acid and neutral glycosphingolipids (0.01-1 mumol) were completely methylated in high yields and with little formation of by-products in 10 min at room temperature, using methyl sulphoxide, methyl iodide, and powdered NaOH. Re-methylation of methylated and LiAlH4-reduced gangliosides gave a new derivative that was useful for the analysis of gangliosides by mass spectrometry.

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