Effect of Leaves Extract Levels on In Vitro Ruminal Fermentation, Microbial Population, Methane Production, and Fatty Acid Biohydrogenation

Overview

Journal Molecules

Publisher MDPI

Specialty Biology

Date 2022 Jul 9

PMID 35807533

Authors

Bogumila Nowak

Barbara Moniuszko-Szajwaj

Maria Skorupka

Julia Puchalska

Martyna Kozlowska

Jan Bocianowski

Pawel Antoni Kolodziejski

Malgorzata Szumacher-Strabel

Amlan Kumar Patra

Anna Stochmal

Adam Cieslak

Affiliations

Soon will be listed here.

Abstract

Paulownia is a fast-growing tree that produces a huge mass of leaves as waste that can be used as a feed source for ruminants. The previous study showed that phenolic compounds were the most active biological substances in leaves, which affected the ruminal parameters and methane concentration. However, there are no scientific reports on the leaves extract (PLE) containing phenolic compounds for their mode of action in the rumen. Phenolics constituted the main group of bioactive compounds in PLE (84.4 mg/g dry matter). PLE lowered the concentration of ammonia, modulated the VFA profile in the ruminal fluid, and decreased methane production. The PLE caused a significant reduction of in vitro dry matter degradability, reduced the number of methanogens and protozoa, and affected selected bacteria populations. PLE had a promising effect on the fatty acid profile in the ruminal fluid. as a new dietary component or its extract as a feed additive may be used to mitigate ruminal methanogenesis, resulting in environmental protection and reducing ruminal biohydrogenation, improving milk and meat quality.

Citing Articles

Influence of and aqueous extract on cattle ruminal gas production and degradability .

Elghandour M, Acosta-Lozano N, Alvarado T, Castillo-Lopez E, Cipriano-Salazar M, Barros-Rodriguez M Front Vet Sci. 2023; 10:1090729.

PMID: 37266386 PMC: 10230098. DOI: 10.3389/fvets.2023.1090729.

References

Watts N, Amann M, Arnell N, Ayeb-Karlsson S, Beagley J, Belesova K . The 2020 report of The Lancet Countdown on health and climate change: responding to converging crises. Lancet. 2020; 397(10269):129-170. PMC: 7616803. DOI: 10.1016/S0140-6736(20)32290-X. View

Calsamiglia S, Cardozo P, Ferret A, Bach A . Changes in rumen microbial fermentation are due to a combined effect of type of diet and pH. J Anim Sci. 2007; 86(3):702-11. DOI: 10.2527/jas.2007-0146. View

Nigro O, Tuzi A, Tartaro T, Giaquinto A, Vallini I, Pinotti G . Biological effects of verbascoside and its anti-inflammatory activity on oral mucositis: a review of the literature. Anticancer Drugs. 2019; 31(1):1-5. DOI: 10.1097/CAD.0000000000000818. View

Chen L, Liu S, Wang H, Wang M, Yu L . Relative significances of pH and substrate starch level to roles of Streptococcus bovis S1 in rumen acidosis. AMB Express. 2016; 6(1):80. PMC: 5031564. DOI: 10.1186/s13568-016-0248-2. View

Stochmal A, Moniuszko-Szajwaj B, Zuchowski J, Pecio L, Kontek B, Szumacher-Strabel M . Qualitative and Quantitative Analysis of Secondary Metabolites in Morphological Parts of Paulownia Clon In Vitro 112 and Their Anticoagulant Properties in Whole Human Blood. Molecules. 2022; 27(3). PMC: 8840654. DOI: 10.3390/molecules27030980. View

Puchalska J, Szumacher-Strabel M, Patra A, Slusarczyk S, Gao M, Petric D . The Effect of Different Concentrations of Total Polyphenols from Hybrid Leaves on Ruminal Fermentation, Methane Production and Microorganisms. Animals (Basel). 2021; 11(10). PMC: 8532658. DOI: 10.3390/ani11102843. View

Li M, Penner G, Hernandez-Sanabria E, Oba M, Guan L . Effects of sampling location and time, and host animal on assessment of bacterial diversity and fermentation parameters in the bovine rumen. J Appl Microbiol. 2009; 107(6):1924-34. DOI: 10.1111/j.1365-2672.2009.04376.x. View

Patra A, Saxena J . A new perspective on the use of plant secondary metabolites to inhibit methanogenesis in the rumen. Phytochemistry. 2010; 71(11-12):1198-222. DOI: 10.1016/j.phytochem.2010.05.010. View

Potu R, AbuGhazaleh A, Hastings D, Jones K, Ibrahim S . The effect of lipid supplements on ruminal bacteria in continuous culture fermenters varies with the fatty acid composition. J Microbiol. 2011; 49(2):216-23. DOI: 10.1007/s12275-011-0365-1. View

10.

Zhang T, Mu Y, Zhang R, Xue Y, Guo C, Qi W . Responsive changes of rumen microbiome and metabolome in dairy cows with different susceptibility to subacute ruminal acidosis. Anim Nutr. 2022; 8(1):331-340. PMC: 8718735. DOI: 10.1016/j.aninu.2021.10.009. View

11.

Yu Y, Lee C, Kim J, Hwang S . Group-specific primer and probe sets to detect methanogenic communities using quantitative real-time polymerase chain reaction. Biotechnol Bioeng. 2005; 89(6):670-9. DOI: 10.1002/bit.20347. View

12.

Mannelli F, Cappucci A, Pini F, Pastorelli R, Decorosi F, Giovannetti L . Effect of different types of olive oil pomace dietary supplementation on the rumen microbial community profile in Comisana ewes. Sci Rep. 2018; 8(1):8455. PMC: 5981327. DOI: 10.1038/s41598-018-26713-w. View

13.

Zhang Y, Liu K, Hao X, Xin H . The relationships between odd- and branched-chain fatty acids to ruminal fermentation parameters and bacterial populations with different dietary ratios of forage and concentrate. J Anim Physiol Anim Nutr (Berl). 2016; 101(6):1103-1114. DOI: 10.1111/jpn.12602. View

14.

Clay N, Garnett T, Lorimer J . Dairy intensification: Drivers, impacts and alternatives. Ambio. 2019; 49(1):35-48. PMC: 6888798. DOI: 10.1007/s13280-019-01177-y. View

15.

Denman S, McSweeney C . Development of a real-time PCR assay for monitoring anaerobic fungal and cellulolytic bacterial populations within the rumen. FEMS Microbiol Ecol. 2006; 58(3):572-82. DOI: 10.1111/j.1574-6941.2006.00190.x. View

16.

Wang R, Cao W, Cerniglia C . PCR detection of Ruminococcus spp. in human and animal faecal samples. Mol Cell Probes. 1997; 11(4):259-65. DOI: 10.1006/mcpr.1997.0111. View

17.

Pan J, Yuan C, Lin C, Jia Z, Zheng R . Pharmacological activities and mechanisms of natural phenylpropanoid glycosides. Pharmazie. 2003; 58(11):767-75. View

18.

Newbold C, de la Fuente G, Belanche A, Ramos-Morales E, McEwan N . The Role of Ciliate Protozoa in the Rumen. Front Microbiol. 2015; 6:1313. PMC: 4659874. DOI: 10.3389/fmicb.2015.01313. View

19.

Cardozo P, Calsamiglia S, Ferret A, Kamel C . Screening for the effects of natural plant extracts at different pH on in vitro rumen microbial fermentation of a high-concentrate diet for beef cattle. J Anim Sci. 2005; 83(11):2572-9. DOI: 10.2527/2005.83112572x. View

20.

McAllister T, Bae H, Jones G, Cheng K . Microbial attachment and feed digestion in the rumen. J Anim Sci. 1994; 72(11):3004-18. DOI: 10.2527/1994.72113004x. View