Chemical Composition and Antibacterial Activity of Liquid and Volatile Phase of Essential Oils Against Planktonic and Biofilm-Forming Cells of

Overview

Journal Molecules

Publisher MDPI

Specialty Biology

Date 2022 Jul 9

PMID 35807343

Authors

Malwina Brozyna

Justyna Paleczny

Weronika Kozlowska

Daria Ciecholewska-Jusko

Adam Parfienczyk

Grzegorz Chodaczek

Adam Junka

Affiliations

Soon will be listed here.

Abstract

is an opportunistic pathogen causing life-threatening, hard-to-heal infections associated with the presence of a biofilm. Essential oils (EOs) are promising agents to combat pseudomonal infections because of the alleged antimicrobial activity of their volatile fractions and liquid forms. Therefore, the purpose of this paper was to evaluate the antibacterial efficacy of both volatile and liquid phases of seven EOs (thyme, tea tree, basil, rosemary, eucalyptus, menthol mint, lavender) against biofilm and planktonic cells with the use of a broad spectrum of analytical in vitro methods. According to the study results, the antibacterial activity of EOs in their liquid forms varied from that of the volatile fractions. Overall, liquid and volatile forms of rosemary EO and tea tree EO displayed significant antibiofilm effectiveness. The outcomes indicate that these particular EOs possess the potential to be used in the therapy of infections.

Citing Articles

The Potential Impact of Edible Fruit Extracts on Bacterial Nucleases in Preliminary Research-In Silico and In Vitro Insight.

Szeleszczuk L, Brozyna M, Dudek B, Czarnecki M, Junka A, Czerwinska M Int J Mol Sci. 2025; 26(4).

PMID: 40004218 PMC: 11855197. DOI: 10.3390/ijms26041757.

The chronic wound milieu changes essential oils' antibiofilm activity-an in vitro and larval model study.

Brozyna M, Dudek B, Kozlowska W, Malec K, Paleczny J, Detyna J Sci Rep. 2024; 14(1):2218.

PMID: 38278929 PMC: 10817982. DOI: 10.1038/s41598-024-52424-6.

Identification of Coumarins and Antimicrobial Potential of Ethanolic Extracts of and .

Sousa B, Castro S, Lourido K, Kasper A, Paulino G, Delarmelina C Molecules. 2022; 27(18).

PMID: 36144574 PMC: 9505109. DOI: 10.3390/molecules27185837.

References

Brozyna M, Paleczny J, Kozlowska W, Chodaczek G, Dudek-Wicher R, Felinczak A . The Antimicrobial and Antibiofilm In Vitro Activity of Liquid and Vapour Phases of Selected Essential Oils against . Pathogens. 2021; 10(9). PMC: 8466273. DOI: 10.3390/pathogens10091207. View

Gagne-Thivierge C, Barbeau J, Levesque R, Charette S . A new approach to study attached biofilms and floating communities from Pseudomonas aeruginosa strains of various origins reveals diverse effects of divalent ions. FEMS Microbiol Lett. 2018; 365(14). DOI: 10.1093/femsle/fny155. View

Janssen A, Chin N, Scheffer J, BAERHEIM SVENDSEN A . Screening for antimicrobial activity of some essential oils by the agar overlay technique. Pharm Weekbl Sci. 1986; 8(6):289-92. DOI: 10.1007/BF02280052. View

Poole K . Pseudomonas aeruginosa: resistance to the max. Front Microbiol. 2011; 2:65. PMC: 3128976. DOI: 10.3389/fmicb.2011.00065. View

Sakkas H, Papadopoulou C . Antimicrobial Activity of Basil, Oregano, and Thyme Essential Oils. J Microbiol Biotechnol. 2016; 27(3):429-438. DOI: 10.4014/jmb.1608.08024. View

Dudek-Wicher R, Paleczny J, Kowalska-Krochmal B, Szymczyk-Ziolkowska P, Pachura N, Szumny A . Activity of Liquid and Volatile Fractions of Essential Oils against Biofilm Formed by Selected Reference Strains on Polystyrene and Hydroxyapatite Surfaces. Pathogens. 2021; 10(5). PMC: 8145098. DOI: 10.3390/pathogens10050515. View

Alibi S, Ben Selma W, Ramos-Vivas J, Smach M, Touati R, Boukadida J . Anti-oxidant, antibacterial, anti-biofilm, and anti-quorum sensing activities of four essential oils against multidrug-resistant bacterial clinical isolates. Curr Res Transl Med. 2020; 68(2):59-66. DOI: 10.1016/j.retram.2020.01.001. View

Jiang Y, Wu N, Fu Y, Wang W, Luo M, Zhao C . Chemical composition and antimicrobial activity of the essential oil of Rosemary. Environ Toxicol Pharmacol. 2011; 32(1):63-8. DOI: 10.1016/j.etap.2011.03.011. View

Harmsen M, Yang L, Pamp S, Tolker-Nielsen T . An update on Pseudomonas aeruginosa biofilm formation, tolerance, and dispersal. FEMS Immunol Med Microbiol. 2010; 59(3):253-68. DOI: 10.1111/j.1574-695X.2010.00690.x. View

10.

Hendry E, Worthington T, Conway B, Lambert P . Antimicrobial efficacy of eucalyptus oil and 1,8-cineole alone and in combination with chlorhexidine digluconate against microorganisms grown in planktonic and biofilm cultures. J Antimicrob Chemother. 2009; 64(6):1219-25. DOI: 10.1093/jac/dkp362. View

11.

Azam M, Khan A . Updates on the pathogenicity status of Pseudomonas aeruginosa. Drug Discov Today. 2018; 24(1):350-359. DOI: 10.1016/j.drudis.2018.07.003. View

12.

Abers M, Schroeder S, Goelz L, Sulser A, St Rose T, Puchalski K . Antimicrobial activity of the volatile substances from essential oils. BMC Complement Med Ther. 2021; 21(1):124. PMC: 8053297. DOI: 10.1186/s12906-021-03285-3. View

13.

Junka A, Zywicka A, Szymczyk P, Dziadas M, Bartoszewicz M, Fijalkowski K . A.D.A.M. test (Antibiofilm Dressing's Activity Measurement) - Simple method for evaluating anti-biofilm activity of drug-saturated dressings against wound pathogens. J Microbiol Methods. 2017; 143:6-12. DOI: 10.1016/j.mimet.2017.09.014. View

14.

de Sousa J, de Azeredo G, Torres R, Vasconcelos M, da Conceicao M, de Souza E . Synergies of carvacrol and 1,8-cineole to inhibit bacteria associated with minimally processed vegetables. Int J Food Microbiol. 2012; 154(3):145-51. DOI: 10.1016/j.ijfoodmicro.2011.12.026. View

15.

Yu Z, Tang J, Khare T, Kumar V . The alarming antimicrobial resistance in ESKAPEE pathogens: Can essential oils come to the rescue?. Fitoterapia. 2019; 140:104433. DOI: 10.1016/j.fitote.2019.104433. View

16.

Acs K, Balazs V, Kocsis B, Bencsik T, Boszormenyi A, Horvath G . Antibacterial activity evaluation of selected essential oils in liquid and vapor phase on respiratory tract pathogens. BMC Complement Altern Med. 2018; 18(1):227. PMC: 6064118. DOI: 10.1186/s12906-018-2291-9. View

17.

Bassetti M, Vena A, Croxatto A, Righi E, Guery B . How to manage infections. Drugs Context. 2018; 7:212527. PMC: 5978525. DOI: 10.7573/dic.212527. View

18.

Das S, Horvath B, Safranko S, Jokic S, Szechenyi A, Koszegi T . Antimicrobial Activity of Chamomile Essential Oil: Effect of Different Formulations. Molecules. 2019; 24(23). PMC: 6930572. DOI: 10.3390/molecules24234321. View

19.

Cal K . Aqueous solubility of liquid monoterpenes at 293 K and relationship with calculated log P value. Yakugaku Zasshi. 2006; 126(4):307-9. DOI: 10.1248/yakushi.126.307. View

20.

Amat S, Baines D, Alexander T . A vapour phase assay for evaluating the antimicrobial activities of essential oils against bovine respiratory bacterial pathogens. Lett Appl Microbiol. 2017; 65(6):489-495. DOI: 10.1111/lam.12804. View