SPR-Based Detection of ASF Virus in Cells

Overview

Journal Int J Mol Sci

Publisher MDPI

Specialties Biochemistry
Chemistry
Molecular Biology

Date 2022 Jul 9

PMID 35806467

Authors

Alessandro Capo

Alessia Calabrese

Maciej Frant

Marek Walczak

Anna Szczotka-Bochniarz

Georgios Manessis

Ioannis Bossis

Maria Staiano

Sabato DAuria

Antonio Varriale

Affiliations

Soon will be listed here.

Abstract

African swine fever (ASF) is one of the most dangerous hemorrhagic infectious diseases that affect domestic and wild pigs. Currently, neither a vaccine nor effective treatments are available for this disease. As regards the degree of virulence, ASFV strains can be divided into high, moderate, or low virulence. The main detection methods are based on the use of the polymerase chain reaction (PCR). In order to prevent an uncontrolled spread of ASF, new on-site techniques that can enable the identification of an early-stage disease are needed. We have developed a specific immunological SPR-based assay for ASFV antigen detection directly in liquid samples. The developed assay allows us to detect the presence of ASFV at the dose of 10 HAD/.

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References

Ge S, Li J, Fan X, Liu F, Li L, Wang Q . Molecular Characterization of African Swine Fever Virus, China, 2018. Emerg Infect Dis. 2018; 24(11):2131-2133. PMC: 6199985. DOI: 10.3201/eid2411.181274. View

Alonso C, Miskin J, Hernaez B, Soto L, Canto C, Rodriguez-Crespo I . African swine fever virus protein p54 interacts with the microtubular motor complex through direct binding to light-chain dynein. J Virol. 2001; 75(20):9819-27. PMC: 114554. DOI: 10.1128/JVI.75.20.9819-9827.2001. View

Mighell E, Ward M . African Swine Fever spread across Asia, 2018-2019. Transbound Emerg Dis. 2021; 68(5):2722-2732. DOI: 10.1111/tbed.14039. View

Wozniakowski G, Fraczyk M, Kowalczyk A, Pomorska-Mol M, Niemczuk K, Pejsak Z . Polymerase cross-linking spiral reaction (PCLSR) for detection of African swine fever virus (ASFV) in pigs and wild boars. Sci Rep. 2017; 7:42903. PMC: 5309890. DOI: 10.1038/srep42903. View

Hu J, Wang T, Wang S, Chen M, Wang M, Mu L . Development of a surface plasmon resonance biosensing approach for the rapid detection of porcine circovirus type2 in sample solutions. PLoS One. 2014; 9(10):e111292. PMC: 4213070. DOI: 10.1371/journal.pone.0111292. View

Montagnese C, Barattini P, Giusti A, Balka G, Bruno U, Bossis I . A Diagnostic Device for In-Situ Detection of Swine Viral Diseases: The SWINOSTICS Project. Sensors (Basel). 2019; 19(2). PMC: 6359211. DOI: 10.3390/s19020407. View

Revilla Y, Perez-Nunez D, Richt J . African Swine Fever Virus Biology and Vaccine Approaches. Adv Virus Res. 2018; 100:41-74. DOI: 10.1016/bs.aivir.2017.10.002. View

Nannucci L, Barattini P, Bossis I, Wozniakowski G, Balka G, Pugliese C . Point-of-service Diagnostic Technology for Detection of Swine Viral Diseases. J Vet Res. 2020; 64(1):15-23. PMC: 7105983. DOI: 10.2478/jvetres-2020-0016. View

Gimenez-Lirola L, Mur L, Rivera B, Mogler M, Sun Y, Lizano S . Detection of African Swine Fever Virus Antibodies in Serum and Oral Fluid Specimens Using a Recombinant Protein 30 (p30) Dual Matrix Indirect ELISA. PLoS One. 2016; 11(9):e0161230. PMC: 5017782. DOI: 10.1371/journal.pone.0161230. View

10.

Miao F, Zhang J, Li N, Chen T, Wang L, Zhang F . Rapid and Sensitive Recombinase Polymerase Amplification Combined With Lateral Flow Strip for Detecting African Swine Fever Virus. Front Microbiol. 2019; 10:1004. PMC: 6530510. DOI: 10.3389/fmicb.2019.01004. View

11.

Hubner A, Petersen B, Keil G, Niemann H, Mettenleiter T, Fuchs W . Efficient inhibition of African swine fever virus replication by CRISPR/Cas9 targeting of the viral p30 gene (CP204L). Sci Rep. 2018; 8(1):1449. PMC: 5780455. DOI: 10.1038/s41598-018-19626-1. View

12.

Mazur-Panasiuk N, Walczak M, Juszkiewicz M, Wozniakowski G . The Spillover of African Swine Fever in Western Poland Revealed Its Estimated Origin on the Basis of O174L, K145R, MGF 505-5R and IGR I73R/I329L Genomic Sequences. Viruses. 2020; 12(10). PMC: 7601147. DOI: 10.3390/v12101094. View

13.

Andersson K, Areskoug D, Hardenborg E . Exploring buffer space for molecular interactions. J Mol Recognit. 1999; 12(5):310-5. DOI: 10.1002/(SICI)1099-1352(199909/10)12:5<310::AID-JMR470>3.0.CO;2-5. View

14.

Gallardo C, Soler A, Nieto R, Cano C, Pelayo V, Sanchez M . Experimental Infection of Domestic Pigs with African Swine Fever Virus Lithuania 2014 Genotype II Field Isolate. Transbound Emerg Dis. 2015; 64(1):300-304. DOI: 10.1111/tbed.12346. View

15.

Gomez-Gomez M, Sanchez C, Peransi S, Zurita D, Bellieres L, Recuero S . Photonic Label-Free Biosensors for Fast and Multiplex Detection of Swine Viral Diseases. Sensors (Basel). 2022; 22(3). PMC: 8838678. DOI: 10.3390/s22030708. View

16.

King D, Reid S, Hutchings G, Grierson S, Wilkinson P, Dixon L . Development of a TaqMan PCR assay with internal amplification control for the detection of African swine fever virus. J Virol Methods. 2002; 107(1):53-61. DOI: 10.1016/s0166-0934(02)00189-1. View

17.

Zhou X, Li N, Luo Y, Liu Y, Miao F, Chen T . Emergence of African Swine Fever in China, 2018. Transbound Emerg Dis. 2018; 65(6):1482-1484. DOI: 10.1111/tbed.12989. View

18.

Alejo A, Matamoros T, Guerra M, Andres G . A Proteomic Atlas of the African Swine Fever Virus Particle. J Virol. 2018; 92(23). PMC: 6232493. DOI: 10.1128/JVI.01293-18. View

19.

Hernaez B, Escribano J, Alonso C . African swine fever virus protein p30 interaction with heterogeneous nuclear ribonucleoprotein K (hnRNP-K) during infection. FEBS Lett. 2008; 582(23-24):3275-80. PMC: 2577129. DOI: 10.1016/j.febslet.2008.08.031. View

20.

Griol A, Peransi S, Rodrigo M, Hurtado J, Bellieres L, Ivanova T . Design and Development of Photonic Biosensors for Swine Viral Diseases Detection. Sensors (Basel). 2019; 19(18). PMC: 6766991. DOI: 10.3390/s19183985. View