A Theoretical Analysis of the Effects of Sonication on Differential Absorption Flattening in Suspensions of Membrane Sheets

Overview

Journal Biophys J

Publisher Cell Press

Specialty Biophysics

Date 1987 Apr 1

PMID 3580481

Citations 4

Authors

C L Teeters

J Eccles

B A Wallace

Affiliations

Soon will be listed here.

Abstract

A theoretical analysis is presented for the flattening of absorption and circular dichroic spectra of suspensions of membrane sheets containing proteins. Equations are presented to describe the dependence of this artifact on the size of the sheets. Values for the flattening coefficients QA and QB are calculated both as a function of absorptivity and sheet size. These studies show that sonication in an inadequate procedure for eliminating flattening in these samples.

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References

Wallace B, Mao D . Circular dichroism analyses of membrane proteins: an examination of differential light scattering and absorption flattening effects in large membrane vesicles and membrane sheets. Anal Biochem. 1984; 142(2):317-28. DOI: 10.1016/0003-2697(84)90471-8. View

Gordon D, Holzwarth G . Artifacts in the measured optic activity of membrane suspensions. Arch Biochem Biophys. 1971; 142(2):481-8. DOI: 10.1016/0003-9861(71)90511-x. View

Nabedryk E, Bardin A, Breton J . Further characterization of protein secondary structures in purple membrane by circular dichroism and polarized infrared spectroscopies. Biophys J. 2009; 48(6):873-6. PMC: 1329418. DOI: 10.1016/S0006-3495(85)83848-0. View

Schneider A, Harmatz D . An experimental method correcting for absorption flattening and scattering in suspensions of absorbing particles: circular dichroism and absorption spectra of hemoglobin in situ in red blood cells. Biochemistry. 1976; 15(19):4158-62. DOI: 10.1021/bi00664a004. View

Urry D . Protein conformation in biomembranes: optical rotation and absorption of membrane suspensions. Biochim Biophys Acta. 1972; 265(1):115-68. DOI: 10.1016/0304-4157(72)90021-4. View