Biocompatibility Evaluation of Peo-treated Magnesium Alloy Implants Placed in Rabbit Femur Condyle Notches and Paravertebral Muscles

Overview

Journal Biomater Res

Specialty Biomedical Engineering

Date 2022 Jul 6

PMID 35794655

Authors

Seong Ryoung Kim

Keon Mo Lee

Jin Hong Kim

Young Jin Choi

Han Ick Park

Hwa Chul Jung

Hyung Jin Roh

Jee Hye Lo Han

Joon Rae Kim

Bu-Kyu Lee

Affiliations

Soon will be listed here.

Abstract

Background: Magnesium alloys have been receiving much attention for use in biodegradable metal implants because of their excellent mechanical properties and biocompatibility. However, their rapid breakdown and low bioactivity can cause the implant to lose mechanical integrity before the bone is completely healed. Moreover, hydrogen gas released during degradation can significantly delay the tissue regeneration process. To solve the instability of magnesium alloys, Zn and Ca can be added to improve the mechanical properties and biocompatibility. One other way to improve the mechanical properties of Mg is plasma electrolytic oxidation (PEO), which provides a dense, thick ceramic-like coating on the Mg surface. In this study, high-purity Mg was selected as the control, and Mg-1wt%Zn-0.1wt%Ca alloy and PEO-treated Mg-1wt%Zn-0.1wt%Ca alloy were selected as the test materials; the results of radiographic and histological analyses of their biocompatibility are reported herein.

Materials And Method: Nineteen New Zealand white rabbits were used in the study. Rod-bars (Ø2.7 × 13.6 mm) were placed on both paravertebral muscles, and cannulated screws (Ø2.7x10mm) were placed on both femur condyle notches. Each animal was implanted in all four sites. X-rays were taken at 0, 2, 4, 8, and 12 weeks, micro-CT, and live-CT were taken at 4, 8, and 12 weeks. At weeks 4, 8, and 12, individuals representing each group were selected and sacrificed to prepare specimens for histopathological examination.

Result: The results confirm that in vivo, Mg-1wt%Zn-0.1wt%Ca alloy had higher corrosion resistance than high-purity Mg and safely degraded over time without causing possible side effects (foreign body or inflammatory reactions, etc.). In addition, PEO treatment of Mg-1wt%Zn-0.1wt%Ca alloy had a positive effect on fracture recovery by increasing the bonding area with bone.

Conclusion: Our results suggest that PEO treatment of Mg-1wt%Zn-0.1wt%Ca alloy can be a promising biomaterials in the field of various clinical situations such as orthopedic and maxillofacial surgerys.

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References

Pietak A, Mahoney P, Dias G, Staiger M . Bone-like matrix formation on magnesium and magnesium alloys. J Mater Sci Mater Med. 2007; 19(1):407-15. DOI: 10.1007/s10856-007-3172-9. View

Bernhardt R, Kuhlisch E, Schulz M, Eckelt U, Stadlinger B . Comparison of bone-implant contact and bone-implant volume between 2D-histological sections and 3D-SRµCT slices. Eur Cell Mater. 2012; 23:237-47. DOI: 10.22203/ecm.v023a18. View

Witte F . The history of biodegradable magnesium implants: a review. Acta Biomater. 2010; 6(5):1680-92. DOI: 10.1016/j.actbio.2010.02.028. View

Gu X, Zheng Y, Cheng Y, Zhong S, Xi T . In vitro corrosion and biocompatibility of binary magnesium alloys. Biomaterials. 2008; 30(4):484-98. DOI: 10.1016/j.biomaterials.2008.10.021. View

Witte F, Fischer J, Nellesen J, Vogt C, Vogt J, Donath T . In vivo corrosion and corrosion protection of magnesium alloy LAE442. Acta Biomater. 2009; 6(5):1792-9. DOI: 10.1016/j.actbio.2009.10.012. View

Dang L, Kim Y, Kim S, Lim K, Bode K, Lee M . Radiographic and histologic effects of bone morphogenetic protein-2/hydroxyapatite within bioabsorbable magnesium screws in a rabbit model. J Orthop Surg Res. 2019; 14(1):117. PMC: 6489201. DOI: 10.1186/s13018-019-1143-8. View

Wong H, Yeung K, Lam K, Tam V, Chu P, Luk K . A biodegradable polymer-based coating to control the performance of magnesium alloy orthopaedic implants. Biomaterials. 2009; 31(8):2084-96. DOI: 10.1016/j.biomaterials.2009.11.111. View

Farraro K, Kim K, Woo S, Flowers J, McCullough M . Revolutionizing orthopaedic biomaterials: The potential of biodegradable and bioresorbable magnesium-based materials for functional tissue engineering. J Biomech. 2013; 47(9):1979-86. PMC: 4144980. DOI: 10.1016/j.jbiomech.2013.12.003. View

Staiger M, Pietak A, Huadmai J, Dias G . Magnesium and its alloys as orthopedic biomaterials: a review. Biomaterials. 2005; 27(9):1728-34. DOI: 10.1016/j.biomaterials.2005.10.003. View

10.

Webster T, Ejiofor J . Increased osteoblast adhesion on nanophase metals: Ti, Ti6Al4V, and CoCrMo. Biomaterials. 2004; 25(19):4731-9. DOI: 10.1016/j.biomaterials.2003.12.002. View

11.

Paital S, Dahotre N . Laser surface treatment for porous and textured Ca-P bio-ceramic coating on Ti-6Al-4V. Biomed Mater. 2008; 2(4):274-81. DOI: 10.1088/1748-6041/2/4/011. View

12.

Razavi M, Fathi M, Savabi O, Vashaee D, Tayebi L . Improvement of biodegradability, bioactivity, mechanical integrity and cytocompatibility behavior of biodegradable mg based orthopedic implants using nanostructured Bredigite (Ca7MgSi 4O 16) bioceramic coated via ASD/EPD technique. Ann Biomed Eng. 2014; 42(12):2537-50. DOI: 10.1007/s10439-014-1084-7. View

13.

Fischerauer S, Kraus T, Wu X, Tangl S, Sorantin E, Hanzi A . In vivo degradation performance of micro-arc-oxidized magnesium implants: a micro-CT study in rats. Acta Biomater. 2012; 9(2):5411-20. DOI: 10.1016/j.actbio.2012.09.017. View

14.

Witte F, Kaese V, Haferkamp H, Switzer E, Meyer-Lindenberg A, Wirth C . In vivo corrosion of four magnesium alloys and the associated bone response. Biomaterials. 2004; 26(17):3557-63. DOI: 10.1016/j.biomaterials.2004.09.049. View

15.

Razavi M, Fathi M, Savabi O, Vashaee D, Tayebi L . In vivo biocompatibility of Mg implants surface modified by nanostructured merwinite/PEO. J Mater Sci Mater Med. 2015; 26(5):184. DOI: 10.1007/s10856-015-5514-3. View

16.

Jacobs J, Gilbert J, Urban R . Corrosion of metal orthopaedic implants. J Bone Joint Surg Am. 1998; 80(2):268-82. DOI: 10.2106/00004623-199802000-00015. View

17.

Lutolf M, Hubbell J . Synthetic biomaterials as instructive extracellular microenvironments for morphogenesis in tissue engineering. Nat Biotechnol. 2005; 23(1):47-55. DOI: 10.1038/nbt1055. View

18.

Chung I, Yoo C, Lee E, Ihm J, Park C, Lim J . Postoperative stability after sagittal split ramus osteotomies for a mandibular setback with monocortical plate fixation or bicortical screw fixation. J Oral Maxillofac Surg. 2008; 66(3):446-52. DOI: 10.1016/j.joms.2007.06.643. View

19.

Stevens M, George J . Exploring and engineering the cell surface interface. Science. 2005; 310(5751):1135-8. DOI: 10.1126/science.1106587. View

20.

Kirkland N, Birbilis N, Staiger M . Assessing the corrosion of biodegradable magnesium implants: a critical review of current methodologies and their limitations. Acta Biomater. 2011; 8(3):925-36. DOI: 10.1016/j.actbio.2011.11.014. View