Identification of Key Pathways Involved in White Strain of Extracts-Induced Cell Death of Human Hepatoma Hep3B Cells by Next Generation Sequencing

Overview

Journal Front Pharmacol

Date 2022 Jul 5

PMID 35784738

Authors

Wei-Sung Li

Kun-Tsung Denzel Lee

Li-Yun Chen

Bang-Jau You

Hong-Zin Lee

Affiliations

Soon will be listed here.

Abstract

White strain of is named as white genius mushroom (WGM) and is a popular food in Taiwan. We have confirmed the cytotoxicity of WGM extracts on human Hep3B liver cancer cells. A total of 8711 significantly differential genes were identified through large-scale transcriptome sequencing. According to the KEGG pathway enrichment analysis, autophagy, mitophagy and apoptosis pathways were identified as significant in WGM extracts-treated cells. WGM extracts induced a dose-dependent generation of reactive oxygen species (ROS) and membrane-enclosed vacuoles in Hep3B cells. The inhibition of ROS by the ROS scavengers blocked the induction of cell death and vacuoles formation. We suggested that the cell death and membrane-enclosed vacuoles induced by WGM extracts are dependent on ROS production in Hep3B cells. (2,6)-3,7,11,15,19,23,27,31,35-Nonamethylhexatriaconta-2,6,34-triene-1,11,15,19,23,27,31-heptol and (18:2) lysophosphatidylcholine were identified in WGM extracts. In addition to being a very popular edible mushrooms, WGM may be developed into a dietary supplement or dietary chemopreventive agent for the cancer treatment.

Citing Articles

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Transcriptome analysis revealed the role of mTOR and MAPK signaling pathways in the white strain of extracts-induced cell death of human hepatoma Hep3B cells.

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