The Prognostic Value of LncRNA MCM3AP-AS1 on Clinical Outcomes in Various Cancers: A Meta- and Bioinformatics Analysis

Overview

Journal Dis Markers

Publisher Wiley

Specialty Biochemistry

Date 2022 Jul 5

PMID 35783010

Authors

Liangyin Fu

Guangming Zhang

Yongfeng Wang

Tingting Lu

Bin Liu

Yajun Jiao

Haizhong Ma

Shixun Ma

Kehu Yang

Hui Cai

Affiliations

Soon will be listed here.

Abstract

Background: MCM3AP antisense RNA 1 (MCM3AP-AS1) is a newly identified potential tumor biomarker. Nevertheless, the prognostic value of MCM3AP-AS1 in cancer has been inconsistent in the available studies. We performed this meta-analysis to identify the prognostic role of MCM3AP-AS1 in various cancers.

Methods: We searched PubMed, Web of Science, EMBASE, and the Cochrane Library databases to screen relevant studies. Hazard ratios (HR) or odds ratios (OR) and corresponding 95% confidence intervals (CI) were used to evaluate the relationship between aberrant MCM3AP-AS1 expression and survival and clinicopathological features (CFS) of cancer patients. A meta-analysis was performed using STATA 12.0 software. Additionally, results were validated by an online database based on The Cancer Genome Atlas (TCGA). Subsequently, we analyzed the MCM3AP-AS1-related genes and molecular mechanisms based on the MEM database.

Results: Our results showed that overexpression of MCM3AP-AS1 was related to poor overall survival (OS) (HR = 2.00, 95% CI, 1.52-2.64, < 0.001) and relapse-free survival (RFS) (HR = 3.28, 95% CI 1.56-6.88, = 0.002). In addition, MCM3AP-AS1 overexpression was associated with TNM stage, differentiation grade, and lymph node metastasis, but not significantly with age, gender, and tumor size. In addition, MCM3AP-AS1 overexpression was verified by the GEPIA online database to be associated with poorer survival. The further functional investigation suggested that MCM3AP-AS1 may be involved in several cancer-related pathways.

Conclusions: The overexpression of MCM3AP-AS1 was related to poor survival and CFS. MCM3AP-AS1 may be considered a novel prognostic marker and therapeutic target in various cancers.

References

Luchini C, Veronese N, Nottegar A, Shin J, Gentile G, Granziol U . Assessing the quality of studies in meta-research: Review/guidelines on the most important quality assessment tools. Pharm Stat. 2020; 20(1):185-195. DOI: 10.1002/pst.2068. View

Li X, Lv J, Liu S . MCM3AP-AS1 KD Inhibits Proliferation, Invasion, and Migration of PCa Cells via DNMT1/DNMT3 (A/B) Methylation-Mediated Upregulation of NPY1R. Mol Ther Nucleic Acids. 2020; 20:265-278. PMC: 7078492. DOI: 10.1016/j.omtn.2020.01.016. View

Xue M, Zhuo Y, Shan B . MicroRNAs, Long Noncoding RNAs, and Their Functions in Human Disease. Methods Mol Biol. 2017; 1617:1-25. DOI: 10.1007/978-1-4939-7046-9_1. View

Chen K, Shen D, Tan L, Lai D, Han Y, Gu Y . A Pan-Cancer Analysis Reveals the Prognostic and Immunotherapeutic Value of ALKBH7. Front Genet. 2022; 13:822261. PMC: 8873580. DOI: 10.3389/fgene.2022.822261. View

Siegel R, Miller K, Fuchs H, Jemal A . Cancer Statistics, 2021. CA Cancer J Clin. 2021; 71(1):7-33. DOI: 10.3322/caac.21654. View

Yao L, Sun R, Chen Y, Wang Q, Wei D, Wang X . The quality of evidence in Chinese meta-analyses needs to be improved. J Clin Epidemiol. 2016; 74:73-9. DOI: 10.1016/j.jclinepi.2016.01.003. View

Li S, Zhu Y, Wei C, Li C, Chen W, Jiang S . Identification of Molecular Correlations Between DHRS4 and Progressive Neurodegeneration in Amyotrophic Lateral Sclerosis By Gene Co-Expression Network Analysis. Front Immunol. 2022; 13:874978. PMC: 9035787. DOI: 10.3389/fimmu.2022.874978. View

Qiu L, Ma Y, Yang Y, Ren X, Wang D, Jia X . Pro-Angiogenic and Pro-Inflammatory Regulation by lncRNA MCM3AP-AS1-Mediated Upregulation of DPP4 in Clear Cell Renal Cell Carcinoma. Front Oncol. 2020; 10:705. PMC: 7344272. DOI: 10.3389/fonc.2020.00705. View

Lu F, Song Y, Cui S, Zhao H, Chen Y, Du H . LncRNA MIAT promotes the proliferation, migration, and invasion of melanoma cells through recruiting TCF12 and activating NFAT5. Am J Transl Res. 2021; 13(11):12588-12600. PMC: 8661192. View

10.

Zhou X, Shen G, Ren D, Guo X, Han J, Guo Q . Expression and clinical prognostic value of CYB561 in breast cancer. J Cancer Res Clin Oncol. 2022; 148(8):1879-1892. DOI: 10.1007/s00432-022-03928-z. View

11.

Luo H, Zhang Y, Qin G, Jiang B, Miao L . LncRNA MCM3AP-AS1 sponges miR-148a to enhance cell invasion and migration in small cell lung cancer. BMC Cancer. 2021; 21(1):820. PMC: 8283830. DOI: 10.1186/s12885-021-08365-8. View

12.

Quinn J, Chang H . Unique features of long non-coding RNA biogenesis and function. Nat Rev Genet. 2015; 17(1):47-62. DOI: 10.1038/nrg.2015.10. View

13.

Liang M, Jia J, Chen L, Wei B, Guan Q, Ding Z . LncRNA MCM3AP-AS1 promotes proliferation and invasion through regulating miR-211-5p/SPARC axis in papillary thyroid cancer. Endocrine. 2019; 65(2):318-326. DOI: 10.1007/s12020-019-01939-4. View

14.

Sung H, Ferlay J, Siegel R, Laversanne M, Soerjomataram I, Jemal A . Global Cancer Statistics 2020: GLOBOCAN Estimates of Incidence and Mortality Worldwide for 36 Cancers in 185 Countries. CA Cancer J Clin. 2021; 71(3):209-249. DOI: 10.3322/caac.21660. View

15.

Hou C, Wang X, Du B . lncRNA MCM3AP-AS1 promotes the development of oral squamous cell carcinoma by inhibiting miR-363-5p. Exp Ther Med. 2020; 20(2):978-984. PMC: 7388416. DOI: 10.3892/etm.2020.8738. View

16.

Fang Y, Sun B, Gao J, Huang Y, Wang C . LncRNA SLCO4A1-AS1 Accelerates Growth and Metastasis of Gastric Cancer Regulation of the miR-149/XIAP Axis. Front Oncol. 2021; 11:683256. PMC: 8505954. DOI: 10.3389/fonc.2021.683256. View

17.

Kolde R, Laur S, Adler P, Vilo J . Robust rank aggregation for gene list integration and meta-analysis. Bioinformatics. 2012; 28(4):573-80. PMC: 3278763. DOI: 10.1093/bioinformatics/btr709. View

18.

Qian Y, Shi L, Luo Z . Long Non-coding RNAs in Cancer: Implications for Diagnosis, Prognosis, and Therapy. Front Med (Lausanne). 2020; 7:612393. PMC: 7734181. DOI: 10.3389/fmed.2020.612393. View

19.

Ahn Y, Kim J . Long Non-Coding RNAs as Regulators of Interactions between Cancer-Associated Fibroblasts and Cancer Cells in the Tumor Microenvironment. Int J Mol Sci. 2020; 21(20). PMC: 7589653. DOI: 10.3390/ijms21207484. View

20.

Tang Z, Kang B, Li C, Chen T, Zhang Z . GEPIA2: an enhanced web server for large-scale expression profiling and interactive analysis. Nucleic Acids Res. 2019; 47(W1):W556-W560. PMC: 6602440. DOI: 10.1093/nar/gkz430. View