Inflammation and Fibrosis in the Coal Dust-exposed Lung Described by Confocal Raman Spectroscopy

Overview

Journal PeerJ

Specialties Biology
Environmental Health
General Medicine

Date 2022 Jun 29

PMID 35765591

Authors

Wenyang Wang

Min Mu

Yuanjie Zou

Bing Li

Hangbing Cao

Dong Hu

Xinrong Tao

Affiliations

Soon will be listed here.

Abstract

Background: Coal workers' pneumoconiosis (CWP) is an occupational disease that severely damages the life and health of miners. However, little is known about the molecular and cellular mechanisms changes associated with lung inflammation and fibrosis induced by coal dust. As a non-destructive technique for measuring biological tissue, confocal Raman spectroscopy provides accurate molecular fingerprints of label-free tissues and cells. Here, the progression of lung inflammation and fibrosis in a murine model of CWP was evaluated using confocal Raman spectroscopy.

Methods: A mouse model of CWP was constructed and biochemical analysis in lungs exposed to coal dust after 1 month (CWP-1M) and 3 months (CWP-3M) control tissues (NS) were used by confocal Raman spectroscopy. H&E, immunohistochemical and collagen staining were used to evaluate the histopathology alterations in the lung tissues.

Results: The CWP murine model was successfully constructed, and the mouse lung tissues showed progression of inflammation and fibrosis, accompanied by changes in NF-κB, p53, Bax, and Ki67. Meanwhile, significant differences in Raman bands were observed among the different groups, particularly changes at 1,248, 1,448, 1,572, and 746 cm. These changes were consistent with collagen, Ki67, and Bax levels in the CWP and NS groups.

Conclusion: Confocal Raman spectroscopy represented a novel approach to the identification of the biochemical changes in CWP lungs and provides potential biomarkers of inflammation and fibrosis.

References

Happo M, Hirvonen M, Halinen A, Jalava P, Pennanen A, Sillanpaa M . Chemical compositions responsible for inflammation and tissue damage in the mouse lung by coarse and fine particulate samples from contrasting air pollution in Europe. Inhal Toxicol. 2008; 20(14):1215-31. DOI: 10.1080/08958370802147282. View

Henneberger P, Attfield M . Respiratory symptoms and spirometry in experienced coal miners: effects of both distant and recent coal mine dust exposures. Am J Ind Med. 1997; 32(3):268-74. DOI: 10.1002/(sici)1097-0274(199709)32:3<268::aid-ajim13>3.0.co;2-t. View

Su X, Fang S, Zhang D, Zhang Q, Lu X, Tian J . Raman spectrum reveals Mesenchymal stem cells inhibiting HL60 cells growth. Spectrochim Acta A Mol Biomol Spectrosc. 2017; 177:15-19. DOI: 10.1016/j.saa.2017.01.021. View

Watanabe S, Markov N, Lu Z, Aillon R, Soberanes S, Runyan C . Resetting proteostasis with ISRIB promotes epithelial differentiation to attenuate pulmonary fibrosis. Proc Natl Acad Sci U S A. 2021; 118(20). PMC: 8157939. DOI: 10.1073/pnas.2101100118. View

Lu J, Chen J, Liu C, Zeng Y, Sun Q, Li J . Identification of antibiotic resistance and virulence-encoding factors in Klebsiella pneumoniae by Raman spectroscopy and deep learning. Microb Biotechnol. 2021; 15(4):1270-1280. PMC: 8966003. DOI: 10.1111/1751-7915.13960. View

Lee Y, Yang W, Han J, Kwak D, Kim T, Saba E . Hypericum ascyron L. extract reduces particulate matter-induced airway inflammation in mice. Phytother Res. 2020; 35(3):1621-1633. DOI: 10.1002/ptr.6929. View

Gama V, Swahari V, Schafer J, Kole A, Evans A, Huang Y . The E3 ligase PARC mediates the degradation of cytosolic cytochrome c to promote survival in neurons and cancer cells. Sci Signal. 2014; 7(334):ra67. PMC: 4182917. DOI: 10.1126/scisignal.2005309. View

Yuan J, Wang T, Wang L, Li P, Shen H, Mo Y . Transcriptome-wide association study identifies PSMB9 as a susceptibility gene for coal workers' pneumoconiosis. Environ Toxicol. 2022; 37(8):2103-2114. DOI: 10.1002/tox.23554. View

Finnerty K, Choi J, Lau A, Davis-Gorman G, Diven C, Seaver N . Instillation of coarse ash particulate matter and lipopolysaccharide produces a systemic inflammatory response in mice. J Toxicol Environ Health A. 2007; 70(23):1957-66. DOI: 10.1080/15287390701549229. View

10.

Jee S, Kim H, Bang S, Kim Y, Park H, Paik S . Low-Level Expression of MTUS1 Is Associated with Poor Survival in Patients with Lung Adenocarcinoma. Diagnostics (Basel). 2021; 11(7). PMC: 8306423. DOI: 10.3390/diagnostics11071250. View

11.

Lagace T, Ridgway N . The role of phospholipids in the biological activity and structure of the endoplasmic reticulum. Biochim Biophys Acta. 2013; 1833(11):2499-510. DOI: 10.1016/j.bbamcr.2013.05.018. View

12.

Pucadyil T, Chattopadhyay A . Role of cholesterol in the function and organization of G-protein coupled receptors. Prog Lipid Res. 2006; 45(4):295-333. DOI: 10.1016/j.plipres.2006.02.002. View

13.

Vettore L, Westbrook R, Tennant D . New aspects of amino acid metabolism in cancer. Br J Cancer. 2019; 122(2):150-156. PMC: 7052246. DOI: 10.1038/s41416-019-0620-5. View

14.

Zhang J, Ge W, Yu Q . Structural evaluation of cytochrome c by Raman spectroscopy and its relationship with apoptosis and protein degradation in postmortem bovine muscle. Food Chem. 2021; 362:130189. DOI: 10.1016/j.foodchem.2021.130189. View

15.

Mu M, Li B, Zou Y, Wang W, Cao H, Zhang Y . Coal dust exposure triggers heterogeneity of transcriptional profiles in mouse pneumoconiosis and Vitamin D remedies. Part Fibre Toxicol. 2022; 19(1):7. PMC: 8772169. DOI: 10.1186/s12989-022-00449-y. View

16.

Wang W, Mu M, Zou Y, Li B, Cao H, Hu D . Label-free Raman spectroscopy characterizes signatures of inflammation and fibrosis in the silicosis. Biochem Biophys Res Commun. 2022; 606:114-120. DOI: 10.1016/j.bbrc.2022.03.107. View

17.

Perret J, Plush B, Lachapelle P, Hinks T, Walter C, Clarke P . Coal mine dust lung disease in the modern era. Respirology. 2017; 22(4):662-670. DOI: 10.1111/resp.13034. View

18.

Ng B, Widjaja A, Viswanathan S, Dong J, Chothani S, Lim S . Similarities and differences between IL11 and IL11RA1 knockout mice for lung fibro-inflammation, fertility and craniosynostosis. Sci Rep. 2021; 11(1):14088. PMC: 8266813. DOI: 10.1038/s41598-021-93623-9. View

19.

Okada M, Smith N, Palonpon A, Endo H, Kawata S, Sodeoka M . Label-free Raman observation of cytochrome c dynamics during apoptosis. Proc Natl Acad Sci U S A. 2011; 109(1):28-32. PMC: 3252932. DOI: 10.1073/pnas.1107524108. View

20.

Yue S, Li J, Lee S, Lee H, Shao T, Song B . Cholesteryl ester accumulation induced by PTEN loss and PI3K/AKT activation underlies human prostate cancer aggressiveness. Cell Metab. 2014; 19(3):393-406. PMC: 3969850. DOI: 10.1016/j.cmet.2014.01.019. View