Isolation and Characterization of Lytic Bacteriophages Against Isolates from Human Infections in the North-west of Iran

Overview

Journal Iran J Microbiol

Publisher Tehran University of Medical Sciences

Specialty Infectious Diseases

Date 2022 Jun 29

PMID 35765555

Authors

Raheleh Majdani

Elham Shams Ghahfarokhi

Affiliations

Soon will be listed here.

Abstract

Background And Objectives: With the emergence of antibiotic-resistant strains, using the anti-bacterial potential of bacteriophages could be an effective approach to combat bacterial infections.

Materials And Methods: In this study, after evaluation of antibiotic sensitivity of 20 clinical bacterial isolates of , isolation of lytic phages was performed against 15 isolates using double-layer agar overlay technique. Molecular analysis of isolated phages was carried out using V and III endonucleases. Then, the host range of the phages was evaluated and the phage with the broadest host range (PPaMa1/18) was selected to morphological characteristics by TEM. Also, its one-step growth curve was determined and the stability of the phage to environmental parameters (temperature and pH) was evaluated.

Results: All isolates of were resistant to five antibiotics. In total, 15 phages were successfully isolated from the sewage sources against each of 15 used bacterial isolates. Molecular analysis of the phages showed a high rate of genomic variation. In the morphological analysis of the selected phage (PPaMa1/18) using TEM, an icosahedral head with a size of 90 nm × 75 nm and a long contractile tail (215 nm) were observed which indicated its similarity to the family. The latent period of the PPaMa1/18 was about 20 minutes and its burst size was estimated to be 58 PFU/cell. Also, the PPaMa1/18 phage antibacterial activity was not significantly affected at pH 4-10 and temperature 4-40C°.

Conclusion: Findings demonstrated that isolated bacteriophage (PPaMa1/18) with wide host range, strong lytic effect and high stability can be used as a promising candidate for the treatment of antibiotic-resistant infections caused by

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References

Jamal M, Andleeb S, Jalil F, Imran M, Nawaz M, Hussain T . Isolation and characterization of a bacteriophage and its utilization against multi-drug resistant Pseudomonas aeruginosa-2995. Life Sci. 2017; 190:21-28. DOI: 10.1016/j.lfs.2017.09.034. View

Aloush V, Navon-Venezia S, Seigman-Igra Y, Cabili S, Carmeli Y . Multidrug-resistant Pseudomonas aeruginosa: risk factors and clinical impact. Antimicrob Agents Chemother. 2005; 50(1):43-8. PMC: 1346794. DOI: 10.1128/AAC.50.1.43-48.2006. View

Fu W, Forster T, Mayer O, Curtin J, Lehman S, Donlan R . Bacteriophage cocktail for the prevention of biofilm formation by Pseudomonas aeruginosa on catheters in an in vitro model system. Antimicrob Agents Chemother. 2009; 54(1):397-404. PMC: 2798481. DOI: 10.1128/AAC.00669-09. View

Muller-Merbach M, Kohler K, Hinrichs J . Environmental factors for phage-induced fermentation problems: replication and adsorption of the Lactococcus lactis phage P008 as influenced by temperature and pH. Food Microbiol. 2007; 24(7-8):695-702. DOI: 10.1016/j.fm.2007.04.003. View

Essoh C, Latino L, Midoux C, Blouin Y, Loukou G, NGuetta S . Investigation of a Large Collection of Pseudomonas aeruginosa Bacteriophages Collected from a Single Environmental Source in Abidjan, Côte d'Ivoire. PLoS One. 2015; 10(6):e0130548. PMC: 4482731. DOI: 10.1371/journal.pone.0130548. View

Haq I, Chaudhry W, Akhtar M, Andleeb S, Qadri I . Bacteriophages and their implications on future biotechnology: a review. Virol J. 2012; 9:9. PMC: 3398332. DOI: 10.1186/1743-422X-9-9. View

Ackermann H . 5500 Phages examined in the electron microscope. Arch Virol. 2006; 152(2):227-43. DOI: 10.1007/s00705-006-0849-1. View

Akhwale J, Rohde M, Rohde C, Bunk B, Sproer C, Boga H . Isolation, characterization and analysis of bacteriophages from the haloalkaline lake Elmenteita, Kenya. PLoS One. 2019; 14(4):e0215734. PMC: 6483233. DOI: 10.1371/journal.pone.0215734. View

Pires D, Vilas Boas D, Sillankorva S, Azeredo J . Phage Therapy: a Step Forward in the Treatment of Pseudomonas aeruginosa Infections. J Virol. 2015; 89(15):7449-56. PMC: 4505681. DOI: 10.1128/JVI.00385-15. View

10.

Pires D, Sillankorva S, Faustino A, Azeredo J . Use of newly isolated phages for control of Pseudomonas aeruginosa PAO1 and ATCC 10145 biofilms. Res Microbiol. 2011; 162(8):798-806. DOI: 10.1016/j.resmic.2011.06.010. View

11.

El Didamony G, Askora A, Shehata A . Isolation and characterization of T7-like lytic bacteriophages infecting multidrug resistant Pseudomonas aeruginosa isolated from Egypt. Curr Microbiol. 2015; 70(6):786-91. DOI: 10.1007/s00284-015-0788-8. View

12.

Snyder L, Loman N, Faraj L, Levi K, Weinstock G, Boswell T . Epidemiological investigation of Pseudomonas aeruginosa isolates from a six-year-long hospital outbreak using high-throughput whole genome sequencing. Euro Surveill. 2013; 18(42). DOI: 10.2807/1560-7917.es2013.18.42.20611. View

13.

Wright A, Hawkins C, Anggard E, Harper D . A controlled clinical trial of a therapeutic bacteriophage preparation in chronic otitis due to antibiotic-resistant Pseudomonas aeruginosa; a preliminary report of efficacy. Clin Otolaryngol. 2009; 34(4):349-57. DOI: 10.1111/j.1749-4486.2009.01973.x. View

14.

Chan B, Abedon S, Loc-Carrillo C . Phage cocktails and the future of phage therapy. Future Microbiol. 2013; 8(6):769-83. DOI: 10.2217/fmb.13.47. View

15.

Lambert P . Mechanisms of antibiotic resistance in Pseudomonas aeruginosa. J R Soc Med. 2002; 95 Suppl 41:22-6. PMC: 1308633. View

16.

Cao Z, Zhang J, Niu Y, Cui N, Ma Y, Cao F . Isolation and characterization of a "phiKMV-like" bacteriophage and its therapeutic effect on mink hemorrhagic pneumonia. PLoS One. 2015; 10(1):e0116571. PMC: 4304800. DOI: 10.1371/journal.pone.0116571. View

17.

Henry M, Debarbieux L . Tools from viruses: bacteriophage successes and beyond. Virology. 2012; 434(2):151-61. DOI: 10.1016/j.virol.2012.09.017. View

18.

Cui X, You J, Sun L, Yang X, Zhang T, Huang K . Characterization of Pseudomonas aeruginosa Phage C11 and Identification of Host Genes Required for Virion Maturation. Sci Rep. 2016; 6:39130. PMC: 5175280. DOI: 10.1038/srep39130. View

19.

Clark J, March J . Bacteriophages and biotechnology: vaccines, gene therapy and antibacterials. Trends Biotechnol. 2006; 24(5):212-8. DOI: 10.1016/j.tibtech.2006.03.003. View

20.

Jurczak-Kurek A, Gasior T, Nejman-Falenczyk B, Bloch S, Dydecka A, Topka G . Biodiversity of bacteriophages: morphological and biological properties of a large group of phages isolated from urban sewage. Sci Rep. 2016; 6:34338. PMC: 5048108. DOI: 10.1038/srep34338. View