and Regulate ω-3 Fatty Acid Desaturase Involved in ABA-Mediated Suberization in the Russet Skin of a Mutant of 'Dangshansuli' ( Rehd.)

Overview

Journal Front Plant Sci

Date 2022 Jun 27

PMID 35755695

Authors

Qi Wang

Yaping Liu

Xinyi Wu

Lindu Wang

Jinchao Li

Minchen Wan

Bin Jia

Zhenfeng Ye

Lun Liu

Xiaomei Tang

Shutian Tao

Liwu Zhu

Wei Heng

Affiliations

Soon will be listed here.

Abstract

Russeting, a disorder of pear fruit skin, is mainly caused by suberin accumulation on the inner part of the outer epidermal cell layers. ABA was identified as a crucial phytohormone in suberification. Here, we demonstrated that the ABA content in russet pear skin was higher than in green skin. Then, ABA was applied to explore the changes in phenotype and suberin composition coupled with RNA-Seq and metabolomics to investigate the probably regulatory pathway of ABA-mediated suberification. The results showed that ABA treatment increased the expression of ω-3 fatty acid desaturase (FAD) and the content of α-linolenic acid. We identified 17 PbFADs in white pear, and the expression of was induced by ABA. In addition, the role of in promoting suberification has been demonstrated by overexpression in Arabidopsis and VIGS assays in the fruitlets. GUS staining indicated that the promoter of was activated by ABA. Furthermore, and have been shown to bind to the promoter directly and this was induced by ABA via yeast one-hybrid (Y1H) screening and qRT-PCR. In summary, our study found that ABA induces the expression of and and activates the promoter, contributing to the formation of russet pear skin. Functional identification of key transcription factors will be the goal of future research. These findings reveal the molecular mechanism of ABA-mediated suberization in the russet skin and provide a good foundation for future studies on the formation of russet skin.

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