Electrochemical Determination of Interaction Between SARS-CoV-2 Spike Protein and Specific Antibodies

Overview

Journal Int J Mol Sci

Publisher MDPI

Specialties Biochemistry
Chemistry
Molecular Biology

Date 2022 Jun 24

PMID 35743208

Authors

Maryia Drobysh

Viktorija Liustrovaite

Ausra Baradoke

Alma Rucinskiene

Almira Ramanaviciene

Vilma Ratautaite

Roman Viter

Chien-Fu Chen

Ieva Plikusiene

Urte Samukaite-Bubniene

Rimantas Slibinskas

Evaldas Ciplys

Martynas Simanavicius

Aurelija Zvirbliene

Indre Kucinskaite-Kodze

Arunas Ramanavicius

Affiliations

Soon will be listed here.

Abstract

The serologic diagnosis of coronavirus disease 2019 (COVID-19) and the evaluation of vaccination effectiveness are identified by the presence of antibodies specific to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). In this paper, we present the electrochemical-based biosensing technique for the detection of antibodies specific to the SARS-CoV-2 proteins. Recombinant SARS-CoV-2 spike proteins (rSpike) were immobilised on the surface of a gold electrode modified by a self-assembled monolayer (SAM). This modified electrode was used as a sensitive element for the detection of polyclonal mouse antibodies against the rSpike (anti-rSpike). Electrochemical impedance spectroscopy (EIS) was used to observe the formation of immunocomplexes while cyclic voltammetry (CV) was used for additional analysis of the surface modifications. It was revealed that the impedimetric method and the elaborate experimental conditions are appropriate for the further development of electrochemical biosensors for the serological diagnosis of COVID-19 and/or the confirmation of successful vaccination against SARS-CoV-2.

Citing Articles

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Electrochemical Biosensor for the Determination of Specific Antibodies against SARS-CoV-2 Spike Protein.

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Polyaniline-based electrochemical immunosensor for the determination of antibodies against SARS-CoV-2 spike protein.

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