Identification and Defensive Characterization of from

Overview

Journal Int J Mol Sci

Publisher MDPI

Specialties Biochemistry
Chemistry
Molecular Biology

Date 2022 Jun 24

PMID 35743081

Authors

Bin Liu

Yini Xie

Huanhuan Yin

Zhichun Zhou

Qinghua Liu

Affiliations

Soon will be listed here.

Abstract

is a pioneer species for afforestation timber and oleoresin, while epidemics of pinewood nematode (PWN; ) are causing a serious biotic disaster for in China. Importantly, resistant could leak copious oleoresin terpenoids to build particular defense fronts for survival when attacked by PWN. However, the defense mechanisms regulating this process remain unknown. Here, , a cytochrome P450 monooxygenase gene, was first identified and functionally characterized from resistant following PWN inoculation. The tissue-specific expression pattern and localization of at the transcript and protein levels in resistant indicated that its upregulation in the stem supported its involvement in the metabolic processes of diterpene biosynthesis as a positive part of the defense against PWN attack. Furthermore, overexpression of may enhance the growth and development of plants. In addition, activated the metabolic flux of antioxidases and stress-responsive proteins under drought conditions and improved drought stress tolerance. Our results provide new insights into the favorable role of in diterpene defense mechanisms in response to PWN attack in resistant and provide a novel metabolic engineering scenario to reform the stress tolerance potential of tobacco.

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