Abiotic Stresses Elicitation Potentiates the Productiveness of Cardoon Calli As Bio-Factories for Specialized Metabolites Production

Overview

Journal Antioxidants (Basel)

Date 2022 Jun 24

PMID 35739938

Authors

Rosa DAlessandro

Teresa Docimo

Giulia Graziani

Vincenzo DAmelia

Monica De Palma

Elisa Cappetta

Marina Tucci

Affiliations

Soon will be listed here.

Abstract

Cultivated cardoon ( L. var ) is a Mediterranean traditional food crop. It is adapted to xerothermic conditions and also grows in marginal lands, producing a large biomass rich in phenolic bioactive metabolites and has therefore received attention for pharmaceutical, cosmetic and innovative materials applications. Cardoon cell cultures can be used for the biotechnological production of valuable molecules in accordance with the principles of cellular agriculture. In the current study, we developed an elicitation strategy on leaf-derived cardoon calli for boosting the production of bioactive extracts for cosmetics. We tested elicitation conditions that trigger hyper-accumulation of bioactive phenolic metabolites without compromising calli growth through the application of chilling and salt stresses. We monitored changes in growth, polyphenol accumulation, and antioxidant capability, along with transcriptional variations of key chlorogenic acid and flavonoids biosynthetic genes. At moderate stress intensity and duration (14 days at 50-100 mM NaCl) salt exerted the best eliciting effect by stimulating total phenols and antioxidant power without impairing growth. Hydroalcoholic extracts from elicited cardoon calli with optimal growth and bioactive metabolite accumulation were demonstrated to lack cytotoxicity by MTT assay and were able to stimulate pro-collagen and aquaporin production in dermal cells. In conclusion, we propose a "natural" elicitation system that can be easily and safely employed to boost bioactive metabolite accumulation in cardoon cell cultures and also in pilot-scale cell culture production.

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References

Lattanzio V, Caretto S, Linsalata V, Colella G, Mita G . Signal transduction in artichoke [Cynara cardunculus L. subsp. scolymus (L.) Hayek] callus and cell suspension cultures under nutritional stress. Plant Physiol Biochem. 2018; 127:97-103. DOI: 10.1016/j.plaphy.2018.03.017. View

Zhu J . Cell signaling under salt, water and cold stresses. Curr Opin Plant Biol. 2001; 4(5):401-6. DOI: 10.1016/s1369-5266(00)00192-8. View

Graziani G, Docimo T, De Palma M, Sparvoli F, Izzo L, Tucci M . Changes in Phenolics and Fatty Acids Composition and Related Gene Expression during the Development from Seed to Leaves of Three Cultivated Cardoon Genotypes. Antioxidants (Basel). 2020; 9(11). PMC: 7695130. DOI: 10.3390/antiox9111096. View

Gabotti D, Locatelli F, Cusano E, Baldoni E, Genga A, Pucci L . Cell Suspensions of (var. Futura): Effect of Elicitation on Metabolite Content and Antioxidant Activity. Molecules. 2019; 24(22). PMC: 6891269. DOI: 10.3390/molecules24224056. View

Wang W, Vinocur B, Altman A . Plant responses to drought, salinity and extreme temperatures: towards genetic engineering for stress tolerance. Planta. 2003; 218(1):1-14. DOI: 10.1007/s00425-003-1105-5. View

Eibl R, Meier P, Stutz I, Schildberger D, Huhn T, Eibl D . Plant cell culture technology in the cosmetics and food industries: current state and future trends. Appl Microbiol Biotechnol. 2018; 102(20):8661-8675. PMC: 6153648. DOI: 10.1007/s00253-018-9279-8. View

Ghosh U, Islam M, Siddiqui M, Cao X, Khan M . Proline, a multifaceted signalling molecule in plant responses to abiotic stress: understanding the physiological mechanisms. Plant Biol (Stuttg). 2021; 24(2):227-239. DOI: 10.1111/plb.13363. View

Balestrini R, Brunetti C, Cammareri M, Caretto S, Cavallaro V, Cominelli E . Strategies to Modulate Specialized Metabolism in Mediterranean Crops: From Molecular Aspects to Field. Int J Mol Sci. 2021; 22(6). PMC: 7999214. DOI: 10.3390/ijms22062887. View

Kamarauskaite J, Baniene R, Raudone L, Vilkickyte G, Vainoriene R, Motiekaityte V . Antioxidant and Mitochondria-Targeted Activity of Caffeoylquinic-Acid-Rich Fractions of Wormwood ( L.) and Silver Wormwood ( Nutt.). Antioxidants (Basel). 2021; 10(9). PMC: 8469600. DOI: 10.3390/antiox10091405. View

10.

Taofiq O, Gonzalez-Paramas A, Barreiro M, Ferreira I . Hydroxycinnamic Acids and Their Derivatives: Cosmeceutical Significance, Challenges and Future Perspectives, a Review. Molecules. 2017; 22(2). PMC: 6155946. DOI: 10.3390/molecules22020281. View

11.

Rischer H, Szilvay G, Oksman-Caldentey K . Cellular agriculture - industrial biotechnology for food and materials. Curr Opin Biotechnol. 2020; 61:128-134. DOI: 10.1016/j.copbio.2019.12.003. View

12.

Tito A, Bimonte M, Carola A, De Lucia A, Barbulova A, Tortora A . An oil-soluble extract of Rubus idaeus cells enhances hydration and water homeostasis in skin cells. Int J Cosmet Sci. 2015; 37(6):588-94. DOI: 10.1111/ics.12236. View

13.

Zhu J . Abiotic Stress Signaling and Responses in Plants. Cell. 2016; 167(2):313-324. PMC: 5104190. DOI: 10.1016/j.cell.2016.08.029. View

14.

Benzie I, Strain J . The ferric reducing ability of plasma (FRAP) as a measure of "antioxidant power": the FRAP assay. Anal Biochem. 1996; 239(1):70-6. DOI: 10.1006/abio.1996.0292. View

15.

Farag M, Deavours B, de Fatima A, Naoumkina M, Dixon R, Sumner L . Integrated metabolite and transcript profiling identify a biosynthetic mechanism for hispidol in Medicago truncatula cell cultures. Plant Physiol. 2009; 151(3):1096-113. PMC: 2773099. DOI: 10.1104/pp.109.141481. View

16.

Smetanska I . Production of secondary metabolites using plant cell cultures. Adv Biochem Eng Biotechnol. 2008; 111:187-228. DOI: 10.1007/10_2008_103. View

17.

Wilson S, Roberts S . Recent advances towards development and commercialization of plant cell culture processes for the synthesis of biomolecules. Plant Biotechnol J. 2011; 10(3):249-68. PMC: 3288596. DOI: 10.1111/j.1467-7652.2011.00664.x. View

18.

Papazoglou E . Responses of Cynara cardunculus L. to single and combined cadmium and nickel treatment conditions. Ecotoxicol Environ Saf. 2010; 74(2):195-202. DOI: 10.1016/j.ecoenv.2010.06.026. View

19.

Ramirez-Estrada K, Vidal-Limon H, Hidalgo D, Moyano E, Golenioswki M, Cusido R . Elicitation, an Effective Strategy for the Biotechnological Production of Bioactive High-Added Value Compounds in Plant Cell Factories. Molecules. 2016; 21(2):182. PMC: 6273650. DOI: 10.3390/molecules21020182. View

20.

Magana A, Kamimura N, Soumyanath A, Stevens J, Maier C . Caffeoylquinic acids: chemistry, biosynthesis, occurrence, analytical challenges, and bioactivity. Plant J. 2021; 107(5):1299-1319. PMC: 9084498. DOI: 10.1111/tpj.15390. View