Influenza A in Wild Boars: Viral Circulation in the Emilia-Romagna Region (Northern Italy) Between 2017 and 2022

Overview

Journal Animals (Basel)

Date 2022 Jun 24

PMID 35739929

Authors

Alice Prosperi

Laura Soliani

Elena Canelli

Laura Baioni

Valentina Gabbi

Camilla Torreggiani

Roberta Manfredi

Irene Calanchi

Giovanni Pupillo

Filippo Barsi

Patrizia Bassi

Laura Fiorentini

Matteo Frasnelli

Maria Cristina Fontana

Andrea Luppi

Chiara Chiapponi

Affiliations

Soon will be listed here.

Abstract

A systematic surveillance against influenza A viruses (IAVs) in the population is essential, considering their role as IAV mixing vessels. However, the viral circulation in wild species is poorly investigated in comparison to the knowledge of IAV infection dynamics in domestic pigs. This study investigated the circulation and the genetic diversity of wild boars' IAVs detected in the Emilia-Romagna region (2017-2022). A total of 4605 lung samples were screened via an M gene real-time RT-PCR for SwIAV; positive samples were subtyped by multiplex RT-PCR, and viral isolation was attempted. Isolated strains (3 out of the 17 positives) were fully sequenced to evaluate viral genotypic diversity. H1N1 was the most frequently detected subtype, with identification of H1pdm09N1 and H1avN1. Whole-genome phylogenetic analysis revealed SwIAVs belonging to different genotypes, with different genetic combinations, and highlighted the simultaneous circulation of the same genotypes in both pigs and wild boars, supporting the hypothesis of SwIAV spillover events at the wildlife-livestock interface. This study represents an update on the wild boar SwIAV Italian situation, and the strains' complete genome analysis showed an evolving and interesting situation that deserves further investigation.

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