Frequency of Semen Collection Affects Ram Sperm Cryoresistance

Overview

Journal Animals (Basel)

Date 2022 Jun 24

PMID 35739829

Authors

Cristina Palacin-Martinez

Mercedes Alvarez

Rafael Montes-Garrido

Marta Neila-Montero

Luis Anel-Lopez

Paulino de Paz

Luis Anel

Marta F Riesco

Affiliations

Soon will be listed here.

Abstract

The improvement of frozen-thawed sperm quality has been mostly approached from the view of cryopreservation protocol optimization in terms of cryoprotectant solutions, freezing-thawing rates and antioxidant supplementation, while the impact of sperm collection frequency remains unknown in rams. In this work, a multiparametric study was carried out in cooled and frozen-thawed semen to evaluate sperm quality after different semen collection frequencies during a month: zero sperm collection (0 CW), four sperm collections per week (4 CW), and ten sperm collections per week (10 CW). Traditional analyses have been applied, in combination with novel technologies related to redox balance. Frozen-thawed semen quality showed a significant decrease (p < 0.05) in 0 CW and 10 CW in comparison to 4 CW, concerning motility and kinetics parameters. However, apoptosis showed a significant increase (p < 0.05) in 10 CW in comparison to 0 CW and 4 CW. The employment methods related to redox balance provided us with the definitive probe to ensure the influence of collection frequency on balance redox after thawing. Specifically, glutathione peroxidase (GPX) and superoxide dismutase (SOD) activity showed a significant decrease (p < 0.05) in 10 CW compared to 0 CW and 4 CW. The characterization of alternative strategies to sperm cryopreservation based on consideration of male sexual regimes, could improve the quality of frozen-thawed sperm.

Citing Articles

Effect of Different Thawing Regimes on Cell Kinematics and Organelle Integrity of Nitrogen-Stored Wallachian Ram Spermatozoa.

Ptacek M, Savvulidi F, LeBrun C, Janosikova M, Kenzhebaev T, Omashev K Vet Sci. 2024; 11(12).

PMID: 39728942 PMC: 11680395. DOI: 10.3390/vetsci11120602.

Seminal plasma removal for medium-term preservation of ram sperm at 5 °C.

Neila-Montero M, Alvarez M, Riesco M, Soriano-Ubeda C, Montes-Garrido R, Palacin-Martinez C BMC Vet Res. 2024; 20(1):360.

PMID: 39128999 PMC: 11318144. DOI: 10.1186/s12917-024-04214-5.

The characterization of CellROX™ probes could be a crucial factor in ram sperm quality assessment.

Palacin-Martinez C, Anel-Lopez L, Alvarez M, Neila-Montero M, Montes-Garrido R, Soriano-Ubeda C Front Vet Sci. 2024; 11:1342808.

PMID: 38476170 PMC: 10927726. DOI: 10.3389/fvets.2024.1342808.

Kinematic and Morphometric Assessment of Fresh Semen, before, during and after Mating Period in Brahman Bulls.

Araya-Zuniga I, Sevilla F, Molina-Montero R, Roldan E, Barrientos-Morales M, Silvestre M Animals (Basel). 2024; 14(1).

PMID: 38200863 PMC: 10778289. DOI: 10.3390/ani14010132.

Does Size Matter? Testicular Volume and Its Predictive Ability of Sperm Production in Rams.

Montes-Garrido R, Anel-Lopez L, Riesco M, Neila-Montero M, Palacin-Martinez C, Soriano-Ubeda C Animals (Basel). 2023; 13(20).

PMID: 37893928 PMC: 10603633. DOI: 10.3390/ani13203204.

References

Lecewicz M, Hering D, Kaminski S, Majewska A, Kordan W . Selected qualitative and biochemical parameters of cryopreserved semen of Holstein-Friesian (HF) AI bulls. Pol J Vet Sci. 2015; 18(1):237-9. DOI: 10.1515/pjvs-2015-0030. View

Cassani P, Beconi M, OFlaherty C . Relationship between total superoxide dismutase activity with lipid peroxidation, dynamics and morphological parameters in canine semen. Anim Reprod Sci. 2005; 86(1-2):163-73. DOI: 10.1016/j.anireprosci.2004.06.006. View

Yamamoto Y, Takahashi K . Glutathione peroxidase isolated from plasma reduces phospholipid hydroperoxides. Arch Biochem Biophys. 1993; 305(2):541-5. DOI: 10.1006/abbi.1993.1458. View

Alvarez M, Anel-Lopez L, Boixo J, Chamorro C, Neila-Montero M, Montes-Garrido R . Current challenges in sheep artificial insemination: A particular insight. Reprod Domest Anim. 2019; 54 Suppl 4:32-40. DOI: 10.1111/rda.13523. View

Peris-Frau P, Soler A, Iniesta-Cuerda M, Martin-Maestro A, Sanchez-Ajofrin I, Medina-Chavez D . Sperm Cryodamage in Ruminants: Understanding the Molecular Changes Induced by the Cryopreservation Process to Optimize Sperm Quality. Int J Mol Sci. 2020; 21(8). PMC: 7215299. DOI: 10.3390/ijms21082781. View

Papas M, Catalan J, Fernandez-Fuertes B, Arroyo L, Bassols A, Miro J . Specific Activity of Superoxide Dismutase in Stallion Seminal Plasma Is Related to Sperm Cryotolerance. Antioxidants (Basel). 2019; 8(11). PMC: 6912747. DOI: 10.3390/antiox8110539. View

He Y, Wang K, Zhao X, Zhang Y, Ma Y, Hu J . Differential proteome association study of freeze-thaw damage in ram sperm. Cryobiology. 2015; 72(1):60-8. DOI: 10.1016/j.cryobiol.2015.11.003. View

Pabon D, Meseguer M, Sevillano G, Cobo A, Romero J, Remohi J . A new system of sperm cryopreservation: evaluation of survival, motility, DNA oxidation, and mitochondrial activity. Andrology. 2019; 7(3):293-301. DOI: 10.1111/andr.12607. View

Plaza Davila M, Martin Munoz P, Tapia J, Ferrusola C, Balao da Silva C C, Pena F . Inhibition of Mitochondrial Complex I Leads to Decreased Motility and Membrane Integrity Related to Increased Hydrogen Peroxide and Reduced ATP Production, while the Inhibition of Glycolysis Has Less Impact on Sperm Motility. PLoS One. 2015; 10(9):e0138777. PMC: 4583303. DOI: 10.1371/journal.pone.0138777. View

10.

Agarwal A, Bui A . Oxidation-reduction potential as a new marker for oxidative stress: Correlation to male infertility. Investig Clin Urol. 2017; 58(6):385-399. PMC: 5671957. DOI: 10.4111/icu.2017.58.6.385. View

11.

Anel L, Alvarez M, Martinez-Pastor F, Garcia-Macias V, Anel E, de Paz P . Improvement strategies in ovine artificial insemination. Reprod Domest Anim. 2006; 41 Suppl 2:30-42. DOI: 10.1111/j.1439-0531.2006.00767.x. View

12.

Riesco M, Anel-Lopez L, Neila-Montero M, Palacin-Martinez C, Montes-Garrido R, Alvarez M . ProAKAP4 as Novel Molecular Marker of Sperm Quality in Ram: An Integrative Study in Fresh, Cooled and Cryopreserved Sperm. Biomolecules. 2020; 10(7). PMC: 7408074. DOI: 10.3390/biom10071046. View

13.

Pena F, OFlaherty C, Ortiz Rodriguez J, Cano F, Gaitskell-Phillips G, Gil M . Redox Regulation and Oxidative Stress: The Particular Case of the Stallion Spermatozoa. Antioxidants (Basel). 2019; 8(11). PMC: 6912273. DOI: 10.3390/antiox8110567. View

14.

Marti E, Marti J, Muino-Blanco T, Cebrian-Perez J . Effect of the cryopreservation process on the activity and immunolocalization of antioxidant enzymes in ram spermatozoa. J Androl. 2008; 29(4):459-67. DOI: 10.2164/jandrol.107.003459. View

15.

Peris S, Bilodeau J, Dufour M, Bailey J . Impact of cryopreservation and reactive oxygen species on DNA integrity, lipid peroxidation, and functional parameters in ram sperm. Mol Reprod Dev. 2006; 74(7):878-92. DOI: 10.1002/mrd.20686. View

16.

Dutta S, Majzoub A, Agarwal A . Oxidative stress and sperm function: A systematic review on evaluation and management. Arab J Urol. 2019; 17(2):87-97. PMC: 6600059. DOI: 10.1080/2090598X.2019.1599624. View

17.

Aitken R, Smith T, Jobling M, Baker M, De Iuliis G . Oxidative stress and male reproductive health. Asian J Androl. 2013; 16(1):31-8. PMC: 3901879. DOI: 10.4103/1008-682X.122203. View

18.

Agarwal A, Roychoudhury S, Sharma R, Gupta S, Majzoub A, Sabanegh E . Diagnostic application of oxidation-reduction potential assay for measurement of oxidative stress: clinical utility in male factor infertility. Reprod Biomed Online. 2016; 34(1):48-57. DOI: 10.1016/j.rbmo.2016.10.008. View

19.

Toker M, Alcay S, Gokce E, Ustuner B . Cryopreservation of ram semen with antioxidant supplemented soybean lecithin-based extenders and impacts on incubation resilience. Cryobiology. 2016; 72(3):205-9. DOI: 10.1016/j.cryobiol.2016.05.001. View

20.

Forouzanfar M, Sharafi M, Hosseini S, Ostadhosseini S, Hajian M, Hosseini L . In vitro comparison of egg yolk-based and soybean lecithin-based extenders for cryopreservation of ram semen. Theriogenology. 2009; 73(4):480-7. DOI: 10.1016/j.theriogenology.2009.10.005. View