Protective Effect of Butanolic Fraction of on Fructose-Mediated Metabolic Alterations in Rats

Overview

Journal Metabolites

Publisher MDPI

Date 2022 Jun 23

PMID 35736413

Authors

Syed Nasir Abbas Bukhari

Hira Asif

Alamgeer

Mulazim Hussain Asim

Hafiz Muhammad Irfan

Hasan Ejaz

Mervat A Elsherif

Kashaf Junaid

Affiliations

Soon will be listed here.

Abstract

The present study was conducted with an intent to evaluate the protective effect of butanolic fraction of on fructose mediated metabolic abnormalities in rats. Rats in all groups except control group were fed on 10% fructose for 6 weeks; however, rats in the treated group also received butanolic fraction for the last 3 weeks, along with the fructose. Moreover, phytoconstituents present in butanolic fraction were analyzed using LC-MS. All doses of butanolic fraction profoundly reduce the fructose-induced blood pressure, sympathetic over-activity, and weight gain. Furthermore, butanolic fraction prominently reduces the glucose intolerance and hyperinsulinemia in fructose-fed rats. On treatment with butanolic fraction, oxidative enzymes and the functionality of the aorta was also restored. Phytochemical analysis revealed the presence of several active constituents including bergenin, scopolin, rutinoside, kaempferol, coumaric acid, apigenin, and gingerol. In conclusion, butanolic fraction of has the potential to prevent and recover the fructose-induced metabolic perturbations.

Citing Articles

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PMID: 36676011 PMC: 9862435. DOI: 10.3390/life13010062.

References

Vikrant V, Grover J, Tandon N, Rathi S, Gupta N . Treatment with extracts of Momordica charantia and Eugenia jambolana prevents hyperglycemia and hyperinsulinemia in fructose fed rats. J Ethnopharmacol. 2001; 76(2):139-43. DOI: 10.1016/s0378-8741(01)00218-5. View

Sanchez-Lozada L, Tapia E, Jimenez A, Bautista P, Cristobal M, Nepomuceno T . Fructose-induced metabolic syndrome is associated with glomerular hypertension and renal microvascular damage in rats. Am J Physiol Renal Physiol. 2006; 292(1):F423-9. DOI: 10.1152/ajprenal.00124.2006. View

Da Silva R, de Souza P, Crestani S, Gasparotto Junior A, Boligon A, Athayde M . Hypotensive and diuretic effect of the butanolic soluble fraction of the hydroethanolic extract of bark of Scutia buxifolia Reissek in rats. J Ethnopharmacol. 2015; 172:395-401. DOI: 10.1016/j.jep.2015.07.006. View

Ahangarpour A, Mohammadian M, Dianat M . Antidiabetic effect of hydroalcholic urticadioica leaf extract in male rats with fructose-induced insulin resistance. Iran J Med Sci. 2012; 37(3):181-6. PMC: 3470082. View

Cheng P, Lee H, Lu P, Chen H, Lai C, Sun G . Resveratrol Inhibition of Rac1-Derived Reactive Oxygen Species by AMPK Decreases Blood Pressure in a Fructose-Induced Rat Model of Hypertension. Sci Rep. 2016; 6:25342. PMC: 4853785. DOI: 10.1038/srep25342. View

Wang L, Tu Y, Lian T, Hung J, Yen J, Wu M . Distinctive antioxidant and antiinflammatory effects of flavonols. J Agric Food Chem. 2006; 54(26):9798-804. DOI: 10.1021/jf0620719. View

Mohan M, Jaiswal B, Kasture S . Effect of Solanum torvum on blood pressure and metabolic alterations in fructose hypertensive rats. J Ethnopharmacol. 2009; 126(1):86-9. DOI: 10.1016/j.jep.2009.08.008. View

Maneesai P, Bunbupha S, Kukongviriyapan U, Prachaney P, Tangsucharit P, Kukongviriyapan V . Asiatic acid attenuates renin-angiotensin system activation and improves vascular function in high-carbohydrate, high-fat diet fed rats. BMC Complement Altern Med. 2016; 16:123. PMC: 4849098. DOI: 10.1186/s12906-016-1100-6. View

Deedwania P . Mechanisms of endothelial dysfunction in the metabolic syndrome. Curr Diab Rep. 2003; 3(4):289-92. DOI: 10.1007/s11892-003-0019-8. View

10.

Khan H, Amin H, Ullah A, Saba S, Rafique J, Khan K . Antioxidant and Antiplasmodial Activities of Bergenin and 11-O-Galloylbergenin Isolated from Mallotus philippensis. Oxid Med Cell Longev. 2016; 2016:1051925. PMC: 4779831. DOI: 10.1155/2016/1051925. View

11.

Manh Hung T, Na M, Thuong P, Su N, Sok D, Song K . Antioxidant activity of caffeoyl quinic acid derivatives from the roots of Dipsacus asper Wall. J Ethnopharmacol. 2006; 108(2):188-92. DOI: 10.1016/j.jep.2006.04.029. View

12.

Saleem H, Htar T, Naidu R, Nawawi N, Ahmad I, Ashraf M . Biological, chemical and toxicological perspectives on aerial and roots of Filago germanica (L.) huds: Functional approaches for novel phyto-pharmaceuticals. Food Chem Toxicol. 2018; 123:363-373. DOI: 10.1016/j.fct.2018.11.016. View

13.

Asif H, Alamgeer , Bukhari I, Vohra F, Afzal S, Khan S . Phytochemical analysis of crude extract of and its effect on hypertension and metabolic perturbations in fructose fed rats. Nat Prod Res. 2019; 35(17):2982-2986. DOI: 10.1080/14786419.2019.1679134. View

14.

Softic S, Cohen D, Kahn C . Role of Dietary Fructose and Hepatic De Novo Lipogenesis in Fatty Liver Disease. Dig Dis Sci. 2016; 61(5):1282-93. PMC: 4838515. DOI: 10.1007/s10620-016-4054-0. View

15.

Nakagawa T, Tuttle K, Short R, Johnson R . Hypothesis: fructose-induced hyperuricemia as a causal mechanism for the epidemic of the metabolic syndrome. Nat Clin Pract Nephrol. 2006; 1(2):80-6. DOI: 10.1038/ncpneph0019. View

16.

May J . How does ascorbic acid prevent endothelial dysfunction?. Free Radic Biol Med. 2000; 28(9):1421-9. DOI: 10.1016/s0891-5849(00)00269-0. View

17.

Rosen P, Ohly P, Gleichmann H . Experimental benefit of moxonidine on glucose metabolism and insulin secretion in the fructose-fed rat. J Hypertens Suppl. 1997; 15(1):S31-8. DOI: 10.1097/00004872-199715011-00004. View

18.

Asif H, Alamgeer -, Ahmad M, Alotaibi N, Alharbi K, Bukhari S . Phytochemical analysis and reappraisal of diuretic activity of Delphinium brunonianum Royle and its mode of action in experimental rats. Pak J Pharm Sci. 2021; 33(4(Supplementary)):1833-1838. View

19.

Elkayam A, Mirelman D, Peleg E, Wilchek M, Miron T, Rabinkov A . The effects of allicin on weight in fructose-induced hyperinsulinemic, hyperlipidemic, hypertensive rats. Am J Hypertens. 2003; 16(12):1053-6. DOI: 10.1016/j.amjhyper.2003.07.011. View

20.

Feldeisen S, Tucker K . Nutritional strategies in the prevention and treatment of metabolic syndrome. Appl Physiol Nutr Metab. 2007; 32(1):46-60. DOI: 10.1139/h06-101. View