Antihypertensive Effects of IGTGIPGIW Peptide Purified from : P-eNOS and P-AKT Stimulation in EA.hy926 Cells and Lowering of Blood Pressure in SHR Model

Overview

Journal Mar Drugs

Publisher MDPI

Specialties Biology
Pharmacology

Date 2022 Jun 23

PMID 35736156

Authors

Hyo-Geun Lee

Hyun-Soo Kim

Hyesuck An

Kyunghwa Baek

Jeong Min Lee

Mi-Jin Yim

Seok-Chun Ko

Ji-Yul Kim

Gun-Woo Oh

Jun-Geon Je

Dae-Sung Lee

You-Jin Jeon

Affiliations

Soon will be listed here.

Abstract

The aim of this study was to assess the potential hypertensive effects of the IGTGIPGIW peptide purified from alcalase hydrolysate (HA) for application in the functional food industry. We investigated the antihypertensive effects of IGTGIPGIW in vitro by assessing nitric oxide production in EA.hy926 endothelial cells, which is a major factor affecting vasorelaxation. The potential vasorelaxation effect was evaluated using 4-amino-5-methylamino-2',7'-difluorofluorescein diacetate, a fluorescent stain. IGTGIPGIW significantly increased the expression of endothelial-derived relaxing factors, including endothelial nitric oxide synthase and protein kinase B, in EA.hy926 cells. Furthermore, oral administration of IGTGIPGIW significantly lowered the systolic blood pressure (183.60 ± 1.34 mmHg) and rapidly recovered the diastolic blood pressure (143.50 ± 5.55 mmHg) in the spontaneously hypertensive rat model in vivo. Our results demonstrate the antihypertensive activity of the IGTGIPGIW peptide purified from and indicate its suitability for application in the functional food industry.

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