Effect of Sustained Systemic Administration of Ginger (Z Officinale) Rhizome Extracts on Salivary Flow in Mice

Overview

Journal Int Dent J

Publisher Elsevier

Specialty Dentistry

Date 2022 Jun 20

PMID 35725589

Authors

Chang Yu Kan

Jing Xuan Hng

Andy Goh

Frederick Smales

Eng Lai Tan

Shiming Zhang

Mallikarjuna Rao Pichika

Abhishek Parolia

Affiliations

Soon will be listed here.

Abstract

Objective: This study aimed to evaluate the effect of methanol (70% v/v), ethanol (80% v/v), dimethyl sulfoxide (DMSO; 100% v/v) extracts of ginger rhizome (GR), and 6-shogaol on the pilocarpine-stimulated salivary flow rate in C57BL/6 mice.

Methods: Three extracts of ginger (Zingiber officinale) rhizome prepared by maceration using the respective solvents and 6-shogoal were reconstituted in normal saline with 0.2% DMSO. Thirty C57BL/6 15-week-old mice were divided into 5 groups: Group 1, saline; Group 2, 70% methanol extract; Group 3, 80% ethanol extract; Group 4, 100% DMSO extract; and Group 5, 6-shogaol. The baseline pilocarpine-stimulated salivary flow rate was measured at the age of 15 weeks (15th week), and treatment solutions were administered by intraperitoneal injection from the 16th to 18th week. The stimulated salivary flow rate during treatment weeks was recorded for each group, and its difference with baseline was analysed using paired-sample t test. The change in salivary flow rate between the treatment groups and the control group was analysed using one-way analysis of variance.

Results: Groups 2, 3, 4, and 5 showed a significant increase in salivary flow rate when compared to baseline (P < .05). The increase in salivary flow rate in all 4 treatment groups was significant when compared to the control group (P < .05). Group 4 produced the highest increase in salivary flow rate; however, the differences amongst the treatment groups did not reach statistical significance (P > .05).

Conclusions: All GR extracts (70% methanol, 80% ethanol, 100% DMSO) and 6-shogaol were equally effective in increasing the pilocarpine-stimulated salivary flow rate in C57BL/6 mice when administered systemically as a sustained dose for 3 weeks.

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References

Mirzaii-Dizgah I, Agha-Hosseini F . Unstimulated whole saliva parathyroid hormone in postmenopausal women with xerostomia. J Contemp Dent Pract. 2011; 12(3):196-9. DOI: 10.5005/jp-journals-10024-1034. View

Yamauchi Y, Matsuno T, Omata K, Satoh T . Relationship between hyposalivation and oxidative stress in aging mice. J Clin Biochem Nutr. 2017; 61(1):40-46. PMC: 5525015. DOI: 10.3164/jcbn.16-79. View

Ahmad M, Bhayat A, Zafar M, Al-Samadani K . The Impact of Hyposalivation on Quality of Life (QoL) and Oral Health in the Aging Population of Al Madinah Al Munawarrah. Int J Environ Res Public Health. 2017; 14(4). PMC: 5409645. DOI: 10.3390/ijerph14040445. View

Li Z, Zhao D, Gong B, Xu Y, Sun H, Yang B . Decreased saliva secretion and down-regulation of AQP5 in submandibular gland in irradiated rats. Radiat Res. 2006; 165(6):678-87. DOI: 10.1667/RR3569.1. View

Murakami M, Wei M, Ding W, Zhang Q . Effects of Chinese herbs on salivary fluid secretion by isolated and perfused rat submandibular glands. World J Gastroenterol. 2009; 15(31):3908-15. PMC: 2731253. DOI: 10.3748/wjg.15.3908. View

Baliga M, Haniadka R, Pereira M, Dsouza J, Pallaty P, Bhat H . Update on the chemopreventive effects of ginger and its phytochemicals. Crit Rev Food Sci Nutr. 2011; 51(6):499-523. DOI: 10.1080/10408391003698669. View

Dugasani S, Pichika M, Nadarajah V, Balijepalli M, Tandra S, Korlakunta J . Comparative antioxidant and anti-inflammatory effects of [6]-gingerol, [8]-gingerol, [10]-gingerol and [6]-shogaol. J Ethnopharmacol. 2009; 127(2):515-20. DOI: 10.1016/j.jep.2009.10.004. View

Villa A, Connell C, Abati S . Diagnosis and management of xerostomia and hyposalivation. Ther Clin Risk Manag. 2015; 11:45-51. PMC: 4278738. DOI: 10.2147/TCRM.S76282. View

Affoo R, Foley N, Garrick R, Siqueira W, Martin R . Meta-Analysis of Salivary Flow Rates in Young and Older Adults. J Am Geriatr Soc. 2015; 63(10):2142-51. DOI: 10.1111/jgs.13652. View

10.

Kuraji M, Matsuno T, Satoh T . Astaxanthin affects oxidative stress and hyposalivation in aging mice. J Clin Biochem Nutr. 2016; 59(2):79-85. PMC: 5018570. DOI: 10.3164/jcbn.15-150. View

11.

Young H, Luo Y, Cheng H, Hsieh W, Liao J, Peng W . Analgesic and anti-inflammatory activities of [6]-gingerol. J Ethnopharmacol. 2004; 96(1-2):207-10. DOI: 10.1016/j.jep.2004.09.009. View

12.

Kondo Y, Nakamoto T, Jaramillo Y, Choi S, Catalan M, Melvin J . Functional differences in the acinar cells of the murine major salivary glands. J Dent Res. 2015; 94(5):715-21. PMC: 4502782. DOI: 10.1177/0022034515570943. View

13.

Karna P, Chagani S, Gundala S, Rida P, Asif G, Sharma V . Benefits of whole ginger extract in prostate cancer. Br J Nutr. 2011; 107(4):473-84. PMC: 3426621. DOI: 10.1017/S0007114511003308. View

14.

Hsu P, Yang S, Tsang N, Fan K, Hsieh C, Lin J . Efficacy of Traditional Chinese Medicine in Xerostomia and Quality of Life during Radiotherapy for Head and Neck Cancer: A Prospective Pilot Study. Evid Based Complement Alternat Med. 2016; 2016:8359251. PMC: 5011503. DOI: 10.1155/2016/8359251. View

15.

Siddaraju M, Dharmesh S . Inhibition of gastric H+, K+-ATPase and Helicobacter pylori growth by phenolic antioxidants of Zingiber officinale. Mol Nutr Food Res. 2007; 51(3):324-32. DOI: 10.1002/mnfr.200600202. View

16.

Wu H, Hsieh M, Lo C, Liu C, Sang S, Ho C . 6-Shogaol is more effective than 6-gingerol and curcumin in inhibiting 12-O-tetradecanoylphorbol 13-acetate-induced tumor promotion in mice. Mol Nutr Food Res. 2010; 54(9):1296-306. DOI: 10.1002/mnfr.200900409. View

17.

Chamani G, Zarei M, Mehrabani M, Taghiabadi Y . Evaluation of effects of Zingiber officinale on salivation in rats. Acta Med Iran. 2011; 49(6):336-40. View

18.

Ship J, Nolan N, Puckett S . Longitudinal analysis of parotid and submandibular salivary flow rates in healthy, different-aged adults. J Gerontol A Biol Sci Med Sci. 1995; 50(5):M285-9. DOI: 10.1093/gerona/50a.5.m285. View

19.

Jung M, Lee M, Park H, Oh E, Shin J, Park J . Heat-induced conversion of gingerols to shogaols in ginger as affected by heat type (dry or moist heat), sample type (fresh or dried), temperature and time. Food Sci Biotechnol. 2018; 27(3):687-693. PMC: 6049668. DOI: 10.1007/s10068-017-0301-1. View

20.

Nagler R . Salivary glands and the aging process: mechanistic aspects, health-status and medicinal-efficacy monitoring. Biogerontology. 2004; 5(4):223-33. DOI: 10.1023/B:BGEN.0000038023.36727.50. View