Hybrid Plasmids Containing the AraBAD Genes of Escherichia Coli B/r

Overview

Journal Gene

Specialty Molecular Biology

Date 1978 May 1

PMID 357248

Citations 10

Authors

D A Kaplan

L Greenfield

T Boone

G Wilcox

Affiliations

Soon will be listed here.

Abstract

The DNA fragments generated by restriction endonuclease BamI which contain the araCBAD genes from E.coli B/r have been cloned. The DNA fragments containing ara genes were idenified by a compairson of the BamI fragments of lambdah80dara phages containing different ara deletion mutations. The ara genes were cloned into the plasmid pBR317, a derivative of ColE1. The cloned DNA fragments were analyzed by digestion with pairs of restriction endonucleases to determine the molecular weight of the chimeras and to identify the cloned ara DNA fragments. The cloned ara fragments were also identified by genetic complementation and recombination tests.

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