» Articles » PMID: 35723801

Development of 27 New Microsatellite Markers for the Shanny Lipophrys Pholis

Overview
Journal Mol Biol Rep
Specialty Molecular Biology
Date 2022 Jun 20
PMID 35723801
Authors
Affiliations
Soon will be listed here.
Abstract

Background: The shanny Lipophrys pholis is an intertidal fish that is widely distributed throughout the Northeast Atlantic. Characterized by limited adult mobility and a long pelagic larval duration, the shanny stands as an ideal model to better understand larval dispersal and connectivity dynamics, which are critical parameters with implications for marine conservation and management.

Methods And Results: To this aim, we developed 27 highly polymorphic microsatellite markers and characterized a population of 42 individuals, presenting an average allelic diversity of 20.1 alleles per locus and heterozygosity ranging from 0.619 to 1.

Conclusions: This set of newly developed microsatellite markers will be useful in providing critical insights into the processes which shape L. pholis gene flow and connectivity patterns and can be used to investigate local parentage lineages.

Citing Articles

Isolation and characterization of 17 polymorphic microsatellite loci with tri- and tetra-nucleotide repeat motifs for Ayu (Plecoglossus altivelis) using next-generation sequencing.

Tsukagoshi H, Abe S Mol Biol Rep. 2023; 50(8):7127-7132.

PMID: 37378743 DOI: 10.1007/s11033-023-08566-3.

References
1.
Cowen R, Sponaugle S . Larval dispersal and marine population connectivity. Ann Rev Mar Sci. 2010; 1:443-66. DOI: 10.1146/annurev.marine.010908.163757. View

2.
Galarza J, Carreras-Carbonell J, Macpherson E, Pascual M, Roques S, Turner G . The influence of oceanographic fronts and early-life-history traits on connectivity among littoral fish species. Proc Natl Acad Sci U S A. 2009; 106(5):1473-8. PMC: 2629446. DOI: 10.1073/pnas.0806804106. View

3.
Lima D, Santos M, Ferreira A, Micaelo C, Reis-Henriques M . The use of the shanny Lipophrys pholis for pollution monitoring: a new sentinel species for the northwestern European marine ecosystems. Environ Int. 2007; 34(1):94-101. DOI: 10.1016/j.envint.2007.07.007. View

4.
Trinkaus J . Directional cell movement during early development of the teleost Blennius pholis: II. Transformation of the cells of epithelial clusters into dendritic melanocytes, their dissociation from each other, and their migration to and invasion of the.... J Exp Zool. 1988; 248(1):55-72. DOI: 10.1002/jez.1402480108. View

5.
Harvey J, Lyons B, Page T, Stewart C, Parry J . An assessment of the genotoxic impact of the Sea Empress oil spill by the measurement of DNA adduct levels in selected invertebrate and vertebrate species. Mutat Res. 1999; 441(1):103-14. DOI: 10.1016/s1383-5718(99)00037-6. View