Probing Neuropeptide Volume Transmission In Vivo by Simultaneous Near-Infrared Light-Triggered Release and Optical Sensing

Overview

Journal Angew Chem Int Ed Engl

Specialty Chemistry

Date 2022 Jun 20

PMID 35723610

Authors

Hejian Xiong

Emre Lacin

Hui Ouyang

Aditi Naik

Xueqi Xu

Chen Xie

Jonghae Youn

Blake A Wilson

Krutin Kumar

Tyler Kern

Erin Aisenberg

Daniel Kircher

Xiuying Li

Joseph A Zasadzinski

Celine Mateo

David Kleinfeld

Sabina Hrabetova

Paul A Slesinger

Zhenpeng Qin

Affiliations

Soon will be listed here.

Abstract

Neuropeptides are abundant signaling molecules in the central nervous system. Yet remarkably little is known about their spatiotemporal spread and biological activity. Here, we developed an integrated optical approach using Plasmonic nAnovesicles and cell-based neurotransmitter fluorescent engineered reporter (CNiFER), or PACE, to probe neuropeptide signaling in the mouse neocortex. Small volumes (fL to pL) of exogenously supplied somatostatin-14 (SST) can be rapidly released under near-infrared light stimulation from nanovesicles implanted in the brain and detected by SST2 CNiFERs with nM sensitivity. Our measurements reveal reduced but synchronized SST transmission within 130 μm, and markedly smaller and delayed transmission at longer distances. These measurements enabled a quantitative estimation of the SST loss rate due to peptide degradation and binding. PACE offers a new tool for determining the spatiotemporal scales of neuropeptide volume transmission and signaling in the brain.

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