Distinct Actin-tropomyosin Cofilament Populations Drive the Functional Diversification of Cytoskeletal Myosin Motor Complexes

Overview

Journal iScience

Publisher Cell Press

Date 2022 Jun 20

PMID 35720262

Authors

Theresia Reindl

Sven Giese

Johannes N Greve

Patrick Y Reinke

Igor Chizhov

Sharissa L Latham

Daniel P Mulvihill

Manuel H Taft

Dietmar J Manstein

Affiliations

Soon will be listed here.

Abstract

The effects of N-terminal acetylation of the high molecular weight tropomyosin isoforms Tpm1.6 and Tpm2.1 and the low molecular weight isoforms Tpm1.12, Tpm3.1, and Tpm4.2 on the actin affinity and the thermal stability of actin-tropomyosin cofilaments are described. Furthermore, we show how the exchange of cytoskeletal tropomyosin isoforms and their N-terminal acetylation affects the kinetic and chemomechanical properties of cytoskeletal actin-tropomyosin-myosin complexes. Our results reveal the extent to which the different actin-tropomyosin-myosin complexes differ in their kinetic and functional properties. The maximum sliding velocity of the actin filament as well as the optimal motor density for continuous unidirectional movement, parameters that were previously considered to be unique and invariant properties of each myosin isoform, are shown to be influenced by the exchange of the tropomyosin isoform and the N-terminal acetylation of tropomyosin.

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