Chemical Communication in Artificial Cells: Basic Concepts, Design and Challenges

Overview

Journal Front Mol Biosci

Specialty Biology

Date 2022 Jun 20

PMID 35720123

Authors

Hedi Karoui

Pankaj Singh Patwal

B V V S Pavan Kumar

Nicolas Martin

Affiliations

Soon will be listed here.

Abstract

In the past decade, the focus of bottom-up synthetic biology has shifted from the design of complex artificial cell architectures to the design of interactions between artificial cells mediated by physical and chemical cues. Engineering communication between artificial cells is crucial for the realization of coordinated dynamic behaviours in artificial cell populations, which would have implications for biotechnology, advanced colloidal materials and regenerative medicine. In this review, we focus our discussion on molecular communication between artificial cells. We cover basic concepts such as the importance of compartmentalization, the metabolic machinery driving signaling across cell boundaries and the different modes of communication used. The various studies in artificial cell signaling have been classified based on the distance between sender and receiver cells, just like in biology into autocrine, juxtacrine, paracrine and endocrine signaling. Emerging tools available for the design of dynamic and adaptive signaling are highlighted and some recent advances of signaling-enabled collective behaviours, such as quorum sensing, travelling pulses and predator-prey behaviour, are also discussed.

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References

Chen H, Wang L, Wang S, Li J, Li Z, Lin Y . Construction of Hybrid Bi-microcompartments with Exocytosis-Inspired Behavior toward Fast Temperature-Modulated Transportation of Living Organisms. Angew Chem Int Ed Engl. 2021; 60(38):20795-20802. DOI: 10.1002/anie.202102846. View

Koga S, Williams D, Perriman A, Mann S . Peptide-nucleotide microdroplets as a step towards a membrane-free protocell model. Nat Chem. 2011; 3(9):720-4. DOI: 10.1038/nchem.1110. View

Coste B, Mathur J, Schmidt M, Earley T, Ranade S, Petrus M . Piezo1 and Piezo2 are essential components of distinct mechanically activated cation channels. Science. 2010; 330(6000):55-60. PMC: 3062430. DOI: 10.1126/science.1193270. View

Elani Y, Solvas X, Edel J, Law R, Ces O . Microfluidic generation of encapsulated droplet interface bilayer networks (multisomes) and their use as cell-like reactors. Chem Commun (Camb). 2016; 52(35):5961-4. DOI: 10.1039/c6cc01434h. View

Liu J, Tian L, Qiao Y, Zhou S, Patil A, Wang K . Hydrogel-Immobilized Coacervate Droplets as Modular Microreactor Assemblies. Angew Chem Int Ed Engl. 2020; 59(17):6853-6859. DOI: 10.1002/anie.201916481. View

Jiang S, Caire da Silva L, Ivanov T, Mottola M, Landfester K . Synthetic Silica Nano-Organelles for Regulation of Cascade Reactions in Multi-Compartmentalized Systems. Angew Chem Int Ed Engl. 2021; 61(6):e202113784. PMC: 9306467. DOI: 10.1002/anie.202113784. View

Huang X, Patil A, Li M, Mann S . Design and construction of higher-order structure and function in proteinosome-based protocells. J Am Chem Soc. 2014; 136(25):9225-34. DOI: 10.1021/ja504213m. View

Ip T, Li Q, Brooks N, Elani Y . Manufacture of Multilayered Artificial Cell Membranes through Sequential Bilayer Deposition on Emulsion Templates. Chembiochem. 2021; 22(13):2275-2281. PMC: 8360201. DOI: 10.1002/cbic.202100072. View

Sanford P, Woolston B . Synthetic or natural? Metabolic engineering for assimilation and valorization of methanol. Curr Opin Biotechnol. 2021; 74:171-179. DOI: 10.1016/j.copbio.2021.12.001. View

10.

Gobbo P, Patil A, Li M, Harniman R, Briscoe W, Mann S . Programmed assembly of synthetic protocells into thermoresponsive prototissues. Nat Mater. 2018; 17(12):1145-1153. DOI: 10.1038/s41563-018-0183-5. View

11.

Isaacs F, Hasty J, Cantor C, Collins J . Prediction and measurement of an autoregulatory genetic module. Proc Natl Acad Sci U S A. 2003; 100(13):7714-9. PMC: 164653. DOI: 10.1073/pnas.1332628100. View

12.

Crowe C, Keating C . Liquid-liquid phase separation in artificial cells. Interface Focus. 2018; 8(5):20180032. PMC: 6227770. DOI: 10.1098/rsfs.2018.0032. View

13.

Lentini R, Yeh Martin N, Mansy S . Communicating artificial cells. Curr Opin Chem Biol. 2016; 34:53-61. DOI: 10.1016/j.cbpa.2016.06.013. View

14.

Picollet-Dhahan N, Zuchowska A, Lemeunier I, Le Gac S . Multiorgan-on-a-Chip: A Systemic Approach To Model and Decipher Inter-Organ Communication. Trends Biotechnol. 2021; 39(8):788-810. DOI: 10.1016/j.tibtech.2020.11.014. View

15.

Deshpande S, Brandenburg F, Lau A, Last M, Spoelstra W, Reese L . Spatiotemporal control of coacervate formation within liposomes. Nat Commun. 2019; 10(1):1800. PMC: 6470218. DOI: 10.1038/s41467-019-09855-x. View

16.

Wang X, Tian L, Du H, Li M, Mu W, Drinkwater B . Chemical communication in spatially organized protocell colonies and protocell/living cell micro-arrays. Chem Sci. 2020; 10(41):9446-9453. PMC: 6991169. DOI: 10.1039/c9sc04522h. View

17.

Liu J, Postupalenko V, Lorcher S, Wu D, Chami M, Meier W . DNA-Mediated Self-Organization of Polymeric Nanocompartments Leads to Interconnected Artificial Organelles. Nano Lett. 2016; 16(11):7128-7136. DOI: 10.1021/acs.nanolett.6b03430. View

18.

Bolognesi G, Friddin M, Salehi-Reyhani A, Barlow N, Brooks N, Ces O . Sculpting and fusing biomimetic vesicle networks using optical tweezers. Nat Commun. 2018; 9(1):1882. PMC: 5951844. DOI: 10.1038/s41467-018-04282-w. View

19.

Yang S, Pieters P, Joesaar A, Bogels B, Brouwers R, Myrgorodska I . Light-Activated Signaling in DNA-Encoded Sender-Receiver Architectures. ACS Nano. 2020; 14(11):15992-16002. PMC: 7690052. DOI: 10.1021/acsnano.0c07537. View

20.

Buddingh B, van Hest J . Artificial Cells: Synthetic Compartments with Life-like Functionality and Adaptivity. Acc Chem Res. 2017; 50(4):769-777. PMC: 5397886. DOI: 10.1021/acs.accounts.6b00512. View