Gold Nanoparticle-Functionalized Diatom Biosilica As Label-Free Biosensor for Biomolecule Detection

Overview

Journal Front Bioeng Biotechnol

Date 2022 Jun 17

PMID 35711633

Authors

Tongtong Chen

Feifei Wu

Yang Li

Hussein E Rozan

Xiguang Chen

Chao Feng

Affiliations

Soon will be listed here.

Abstract

Diatom biosilica (DBs) is the cell wall of natural diatom called frustule, which is made of porous hydrogenated amorphous silica possessing periodic micro- to nanoscale features. In this study, a simple, sensitive, and label-free photoluminescence (PL) immune-detection platform based on functionalized diatom frustules was developed. Gold nanoparticles (AuNPs) deposited on poly-dopamine-coated diatom frustules deposition which considerably decreased the intrinsic blue PL intensity of diatom biosilica. Then, goat anti-rabbit immunoglobulin G (IgG) was added to functionalize diatom biosilica-poly-dopamine-AuNPs (DBs-PDA-AuNPs). PL studies revealed that the specific binding with antigen rabbit IgG increased the peak intensity of PL in comparison with the non-complimentary antigen (human IgG). The enhancement in PL intensity of DBs-PDA had a linear correlation with antigen (rabbit IgG) concentration, whose limit of detection (LOD) reached 8 × 10 mg/ml. Furthermore, PL detection based on DBs-PDA-AuNPs showed a high detection sensitivity with the LOD as low as 8 × 10 mg/ml and spread over almost eight orders of magnitude, making it suitable for the sensitive quantitative analysis of immune complex compared with traditional fluorescence immunoassay. Hence, the study proves that the AuNP-functionalized diatom frustules can serve as an effective biosensor platform for label-free PL-based immunoassay.

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