Spontaneous Expression of the Gene of and in Cynomolgus Monkeys Infected with Papillomavirus

Overview

Journal Vet World

Specialty Veterinary Medicine

Date 2022 Jun 14

PMID 35698518

Authors

Huda S Darusman

Sela S Mariya

Isti K Sari

Maulida A Nisa

Kartika Sari

Silmi Mariya

Apon Zaenal Mustopa

Uus Saepuloh

Affiliations

Soon will be listed here.

Abstract

Background And Aim: Cynomolgus monkeys () develop spontaneous infection of Papillomavirus (PV); thus, potentially beneficial for modeling human PV (HPV) infection study. Contrary to human origin, infection in cynomolgus monkeys does not always show evident clinical symptoms of cervical cancer. The absence of cervical cancer clinical symptoms leads us to investigate the molecular mechanism of the HPV infection in cynomolgus monkeys. This study aimed to investigate the messenger ribonucleic acid (mRNA) expression levels of and genes, majorly known as biomarker oncogenesis of PV infection.

Materials And Methods: The polymerase chain reaction (PCR) technique was used with MY11/MY09 primer to screen PV in cynomolgus monkey, further grouped as positive-PV and negative-PV infection groups. Real-time quantitative PCR was also applied to quantify the mRNA expression levels of and genes in animals.

Results: Increased expression of mRNA level of genes was significantly higher in Positive- PV group than negative-PV group. In contrast, the mRNA expression level increased markedly higher in the negative-PV group than in the positive-PV group.

Conclusion: Our study describes the potential of cynomolgus monkeys as a spontaneous oncogenesis model of PV infection-type. However, we used a limited number of cancer genetic markers. So, further study of other genetic markers is required to prove that cervical cancer could be developed naturally in cynomolgus monkeys.

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