Analysis of Carbapenemases Genes of Carbapenem-resistant Isolated from Tehran Heart Center

Overview

Journal Iran J Microbiol

Publisher Tehran University of Medical Sciences

Specialty Infectious Diseases

Date 2022 Jun 6

PMID 35664723

Authors

Leyla Pourgholi

Hadi Farhadinia

Maryam Hosseindokht

Shayan Ziaee

Rahim Nosrati

Marjan Nosrati

Mohammadali Boroumand

Affiliations

Soon will be listed here.

Abstract

Background And Objectives: Emerging of carbapenem-resistant (CRKP) is one of the major concerns among healthcare systems. This study aimed to investigate the antibiotic susceptibility pattern and carbapenemase genes of carbapenemase-producing isolates obtained from Iranian hospitalized patients.

Materials And Methods: This study was performed on 71 CRKP strains isolated from different clinical specimens collected in Tehran Heart Center (Tehran, Iran). A Modified Hodge test (MHT) was done for the detection of carbapenemase-producing . The presence of , , , , and -type carbapenemases was evaluated by the PCR method.

Results: We identified 8.82% (71/805) of isolates as CRKP by MHT test. The antibiotic susceptibility indicated that all isolates were resistant to imipenem, meropenem, cefotaxime, ceftazidime, cefepime, ceftriaxone, cephalothin, ciprofloxacin, and augmentin, and then mostly resistant to aztreonam, cefoxitin, gentamicin, and trimethoprim/sulfamethoxazole with 98.6%, 98.6%, 97.2%, and 94.4%, respectively. The lowest resistance was related to amikacin with 46.5% (33/71 isolates). The level of imipenem MIC for all carbapenem-resistant isolates was estimated ≥32 μg/mL. Among positive isolates for carbapenemase genes, the most frequent gene was . It was found in 48 (67.6%) isolates followed by in 28 (39.4%) isolates. , and genes were identified in 19 (26.8%), 13 (18.3%) and 5 (7.0%) isolates, respectively. These genes were not detected in nine isolates.

Conclusion: The relatively high frequency of some carbapenemase genes suggests major concern about the emergence of isolates containing carbapenem resistance genes as a potential health threat.

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