Prognostic Value of Circulating Endothelial Cells in Glioblastoma Patients: a Pilot Study

Overview

Journal Future Sci OA

Specialty Biotechnology

Date 2022 Jun 6

PMID 35662744

Authors

Maria Angeles Vaz Salgado

Julie Earl

Victor Rodriguez Berrocal

Freddy Salge Arrieta

Ana Gomez

Juan Manuel Sepulveda-Sanchez

Angel Perez-Nunez

Elena Corral de la Fuente

Daniel Lourido

Maria Villamayor

Hector Pian

Alfonso Muriel

Elisabetta Rossi

Rita Zamarchi

Alfredo Carrato

Luis Ley

Affiliations

Soon will be listed here.

Abstract

Aim: Glioblastoma (GB) is an aggressive tumor type and the detection of circulating endothelial cells (CECs) in peripheral blood has been related to angiogenesis.

Materials & Methods: A prospective single-center pilot study of CEC detection at diagnosis in 22 patients with GB was performed, using the US FDA-approved CellSearch system.

Results: A CEC cutoff value was estimated using a receiver operating curve (ROC) and patients were classified into two groups: <40 CEC/4 ml and >40 CEC/4 ml blood. Median overall survival was 25.33 months for group 1 and 8.23 months for group 2 cases (p = 0.02). There was no correlation between CEC and PWI (perfusion-weighted imaging) RM.

Conclusion: CEC detection has a prognostic value in GB cases at diagnosis.

Citing Articles

A Synopsis of Biomarkers in Glioblastoma: Past and Present.

Tataranu L, Turliuc S, Rizea R, Dricu A, Alexandru O, Staicu G Curr Issues Mol Biol. 2024; 46(7):6903-6939.

PMID: 39057054 PMC: 11275428. DOI: 10.3390/cimb46070412.

References

Bidard F, Mathiot C, Degeorges A, Etienne-Grimaldi M, Delva R, Pivot X . Clinical value of circulating endothelial cells and circulating tumor cells in metastatic breast cancer patients treated first line with bevacizumab and chemotherapy. Ann Oncol. 2010; 21(9):1765-1771. DOI: 10.1093/annonc/mdq052. View

Goon P, Lip G, Boos C, Stonelake P, Blann A . Circulating endothelial cells, endothelial progenitor cells, and endothelial microparticles in cancer. Neoplasia. 2006; 8(2):79-88. PMC: 1578513. DOI: 10.1593/neo.05592. View

Bennett I, Guo H, Kountouri N, DAbaco G, Hovens C, Moffat B . Preoperative biomarkers of tumour vascularity are elevated in patients with glioblastoma multiforme. J Clin Neurosci. 2015; 22(11):1802-8. DOI: 10.1016/j.jocn.2015.06.013. View

Rafat N, Beck G, Schulte J, Tuettenberg J, Vajkoczy P . Circulating endothelial progenitor cells in malignant gliomas. J Neurosurg. 2009; 112(1):43-9. DOI: 10.3171/2009.5.JNS081074. View

Ding Y, Gao Z, Jacobson K, Suffredini A . Dexamethasone enhances ATP-induced inflammatory responses in endothelial cells. J Pharmacol Exp Ther. 2010; 335(3):693-702. PMC: 2993554. DOI: 10.1124/jpet.110.171975. View

Ricci-Vitiani L, Pallini R, Biffoni M, Todaro M, Invernici G, Cenci T . Tumour vascularization via endothelial differentiation of glioblastoma stem-like cells. Nature. 2010; 468(7325):824-8. DOI: 10.1038/nature09557. View

Beerepoot L, Mehra N, Vermaat J, Zonnenberg B, Gebbink M, Voest E . Increased levels of viable circulating endothelial cells are an indicator of progressive disease in cancer patients. Ann Oncol. 2003; 15(1):139-45. DOI: 10.1093/annonc/mdh017. View

Willett C, Boucher Y, Tomaso E, Duda D, Munn L, Tong R . Direct evidence that the VEGF-specific antibody bevacizumab has antivascular effects in human rectal cancer. Nat Med. 2004; 10(2):145-7. PMC: 2693485. DOI: 10.1038/nm988. View

Kawaishi M, Fujiwara Y, Fukui T, Kato T, Yamada K, Ohe Y . Circulating endothelial cells in non-small cell lung cancer patients treated with carboplatin and paclitaxel. J Thorac Oncol. 2009; 4(2):208-13. DOI: 10.1097/JTO.0b013e318193030d. View

10.

Reynes G, Vila V, Fleitas T, Reganon E, Font de Mora J, Jorda M . Circulating endothelial cells and procoagulant microparticles in patients with glioblastoma: prognostic value. PLoS One. 2013; 8(7):e69034. PMC: 3726739. DOI: 10.1371/journal.pone.0069034. View

11.

Leon S, Folkerth R, Black P . Microvessel density is a prognostic indicator for patients with astroglial brain tumors. Cancer. 1996; 77(2):362-72. DOI: 10.1002/(SICI)1097-0142(19960115)77:2<362::AID-CNCR20>3.0.CO;2-Z. View

12.

Goon P, Lip G, Stonelake P, Blann A . Circulating endothelial cells and circulating progenitor cells in breast cancer: relationship to endothelial damage/dysfunction/apoptosis, clinicopathologic factors, and the Nottingham Prognostic Index. Neoplasia. 2009; 11(8):771-9. PMC: 2713592. DOI: 10.1593/neo.09490. View

13.

Erdbruegger U, Dhaygude A, Haubitz M, Woywodt A . Circulating endothelial cells: markers and mediators of vascular damage. Curr Stem Cell Res Ther. 2010; 5(4):294-302. DOI: 10.2174/157488810793351721. View

14.

Alexiou G, Vartholomatos G, Karamoutsios A, Batistatou A, Kyritsis A, Voulgaris S . Circulating progenitor cells: a comparison of patients with glioblastoma or meningioma. Acta Neurol Belg. 2012; 113(1):7-11. DOI: 10.1007/s13760-012-0097-y. View

15.

Mancuso P, Gidaro A, Gregato G, Raveane A, Cremonesi P, Quarna J . Circulating endothelial progenitors are increased in COVID-19 patients and correlate with SARS-CoV-2 RNA in severe cases. J Thromb Haemost. 2020; 18(10):2744-2750. PMC: 7436444. DOI: 10.1111/jth.15044. View

16.

Yang Y, Wang Z, Liu H, Zhou Y . Circulating endothelial cells in surgical patients. Cor Vasa. 1992; 34(4):359-64. View

17.

Mancuso P, Colleoni M, Calleri A, Orlando L, Maisonneuve P, Pruneri G . Circulating endothelial-cell kinetics and viability predict survival in breast cancer patients receiving metronomic chemotherapy. Blood. 2006; 108(2):452-9. PMC: 1895485. DOI: 10.1182/blood-2005-11-4570. View

18.

Batchelor T, Sorensen A, Tomaso E, Zhang W, Duda D, Cohen K . AZD2171, a pan-VEGF receptor tyrosine kinase inhibitor, normalizes tumor vasculature and alleviates edema in glioblastoma patients. Cancer Cell. 2007; 11(1):83-95. PMC: 2748664. DOI: 10.1016/j.ccr.2006.11.021. View

19.

Greenfield J, Jin D, Young L, Christos P, Abrey L, Rafii S . Surrogate markers predict angiogenic potential and survival in patients with glioblastoma multiforme. Neurosurgery. 2009; 64(5):819-26. DOI: 10.1227/01.NEU.0000343742.06625.DB. View

20.

Calleri A, Bono A, Bagnardi V, Quarna J, Mancuso P, Rabascio C . Predictive Potential of Angiogenic Growth Factors and Circulating Endothelial Cells in Breast Cancer Patients Receiving Metronomic Chemotherapy Plus Bevacizumab. Clin Cancer Res. 2009; 15(24):7652-7657. DOI: 10.1158/1078-0432.CCR-09-1493. View