A Method to Correct for the Influence of Bovine Serum Albumin-associated Vitamin D Metabolites in Protein Extracts from Neonatal Dried Blood Spots

Overview

Journal BMC Res Notes

Publisher Biomed Central

Specialties Biology
General Medicine

Date 2022 Jun 6

PMID 35659347

Authors

Sanne Grundvad Boelt

Oleguer Plana-Ripoll

Clara Albinana

Bjarni Vilhjalmsson

John J McGrath

Arieh S Cohen

Affiliations

Soon will be listed here.

Abstract

Objective: We developed an assay to measure the concentration of 25 hydroxyvitamin D and D in protein extracts derived from stored neonatal dried blood spots. During this study, we postulated that these samples had been contaminated with exogenous vitamin D metabolites because of the addition of bovine serum albumin (BSA) as part of an extraction step undertaken 7 years earlier. The aim of the current study was to develop methods in order to adjust for this contamination.

Results: We identified between-plate variations in 25 hydroxyvitamin D and D concentrations which suggested the presence of three different BSA batches. Based on repeat extraction (without the addition of BSA) and testing of 395 samples, we developed models to correct for the exogenous 25 hydroxyvitamin D and D The regression models were Diff = - 8.2 + 1.8* Diff for low contamination, Diff = 23.8 + 1.7* Diff for middle contamination, and Diff = 14.3 + 3.0* Diff for high contamination. After these corrections, the three subsamples had comparable distributions within the expected range for both 25 hydroxyvitamin D and D.

Citing Articles

Genetic correlates of vitamin D-binding protein and 25-hydroxyvitamin D in neonatal dried blood spots.

Albinana C, Zhu Z, Borbye-Lorenzen N, Boelt S, Cohen A, Skogstrand K Nat Commun. 2023; 14(1):852.

PMID: 36792583 PMC: 9932173. DOI: 10.1038/s41467-023-36392-5.

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