Development of a Defined Medium for Haemophilus Somnus Isolated from Cattle

Overview

Journal Am J Vet Res

Specialty Veterinary Medicine

Date 1987 Mar 1

PMID 3565890

Citations 28

Authors

T J Inzana

L B Corbeil

Affiliations

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Abstract

Nutritional factors that influence the growth of Haemophilus somnus were examined, and a defined medium was developed. Optimal growth of H somnus in broth occurred under conditions of maximum aeration. Nutritional components required for or enhanced growth of most H somnus isolates in the defined medium included uracil, D-glucose, isotonic NaCl, Na2HPO4, nicotinamide, flavin mononucleotide, pantothenic acid, pyridoxine, and a variety of salts and amino acids. The defined medium supported optimum growth of 18 of 21 isolates of H somnus from cattle.

Citing Articles

Identification and initial characterization of Hfq-associated sRNAs in Histophilus somni strain 2336.

Subhadra B, Cao D, Jensen R, Caswell C, Inzana T PLoS One. 2023; 18(5):e0286158.

PMID: 37220152 PMC: 10204968. DOI: 10.1371/journal.pone.0286158.

The Role of in Histophilus somni Virulence and Biofilm Formation.

Pan Y, Siddaramappa S, Sandal I, Dickerman A, Bandara A, Inzana T Infect Immun. 2020; 89(2).

PMID: 33139386 PMC: 7822144. DOI: 10.1128/IAI.00567-20.

Histophilus somni Survives in Bovine Macrophages by Interfering with Phagosome-Lysosome Fusion but Requires IbpA for Optimal Serum Resistance.

Pan Y, Tagawa Y, Champion A, Sandal I, Inzana T Infect Immun. 2018; 86(12).

PMID: 30201700 PMC: 6246896. DOI: 10.1128/IAI.00365-18.

The Genomic Sequence of the Oral Pathobiont Strain NI1060 Reveals Unique Strategies for Bacterial Competition and Pathogenicity.

Darzi Y, Jiao Y, Hasegawa M, Moon H, Nunez G, Inohara N PLoS One. 2016; 11(7):e0158866.

PMID: 27409077 PMC: 4943601. DOI: 10.1371/journal.pone.0158866.

Detection of antibodies to the biofilm exopolysaccharide of Histophilus somni following infection in cattle by enzyme-linked immunosorbent assay.

Pan Y, Fisher T, Olk C, Inzana T Clin Vaccine Immunol. 2014; 21(10):1463-7.

PMID: 25143338 PMC: 4266353. DOI: 10.1128/CVI.00384-14.