Complete Genome Sequence of a Novel Virus Belonging to the Genus Badnavirus in Jujube (Ziziphus Jujuba Mill.) in China

Overview

Journal Arch Virol

Specialty Microbiology

Date 2022 Jun 3

PMID 35657435

Authors

Baojun Liu

Guoxin Zhang

Danbo Song

Quan Wang

Hong Li

Aixing Gu

Jianyu Bai

Affiliations

Soon will be listed here.

Abstract

The genome sequence of a novel circular DNA virus related to members of the genus Badnavirus was identified in diseased jujube trees by high-throughput sequencing and verified by conventional Sanger sequencing of cloned PCR products. The name "jujube badnavirus WS" (JuBWS) is proposed for this virus. Diseased jujube leaves showed yellow mosaic and malformation symptoms, with round chlorotic spots found on diseased fruit. The genome of this virus has a length of 6450 nt and has a typical badnavirus genomic structure with three open reading frames (ORFs). JuBWS was identified as a novel badnavirus based on nucleotide differences in the RNase (RT + RNase H) coding region of ORF3. The JuBWS sequence showed 70.48-76.41% nucleotide sequence identity to other known badnaviruses, thus meeting the taxonomic criterion for establishing a new species within the genus Badnavirus. This study suggested that the novel badnavirus might be a pathogen associated with jujube mosaic disease, and this will be investigated in the future.

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