Neuroprotective Effect of L-deprenyl on the Expression Level of the Mst1 Gene and Inhibition of Apoptosis in Rat-model Spinal Cord Injury

Overview

Journal Iran J Basic Med Sci

Publisher Mashhad University of Medical Sciences

Specialty General Medicine

Date 2022 Jun 3

PMID 35656451

Authors

Alireza Abdanipour

Ali Nikfar

Mahsa Nikbakht Rad

Iraj Jafari Anarkooli

Mojdeh Mansouri

Affiliations

Soon will be listed here.

Abstract

Objectives: After primary tissue damage as a result of spinal cord injury (SCI), there is a period of secondary damage, which includes several cellular and inflammatory biochemical cascades. As a novel pro-apoptotic kinase, (serine/threonine kinase 4) promotes programmed cell death in an inflammatory disease model. This study aimed to evaluate gene expression levels in rats with spinal cord injury treated with L- deprenyl.

Materials And Methods: The rats were divided into control (contusion), laminectomy, sham-operated (contused rats received 1 ml normal saline intraperitoneal), and treatment (contused rats received 5 mg/kg of L-deprenyl intraperitoneal; once a day for 7 days). The BBB (Basso, Beattie, and Bresnahan) scales were performed to assess motor function following SCI. Rats were sacrificed 28 days after SCI and the spinal cord lesion area was removed. Apoptosis and cavity formation in the spinal cord were determined by H&E staining and TUNEL assay, respectively. The mRNA levels of the , , , and genes were analyzed using real-time quantitative PCR.

Results: The results showed significant improvement in motor function in the L- deprenyl group compared with the untreated group. Histological analysis showed a significant reduction in the number of tunnel-positive cells after injection of L-deprenyl, as well as a decrease in the volume of the cavity. In addition, L-deprenyl treatment increased the expression of the , , and genes, while reducing the expression of the gene in the spinal nerves.

Conclusion: These results suggest that L-deprenyl is a promising treatment for spinal cord injury.

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