Novel Targets in Rectal Cancer by Considering LncRNA-miRNA-mRNA Network in Response to Lactobacillus Acidophilus Consumption: a Randomized Clinical Trial

Overview

Journal Sci Rep

Specialty Science

Date 2022 Jun 2

PMID 35654932

Authors

Zohreh Khodaii

Mahboobeh Mehrabani Natanzi

Solmaz Khalighfard

Maziar Ghandian Zanjan

Maryam Gharghi

Vahid Khori

Taghi Amiriani

Monireh Rahimkhani

Ali Mohammad Alizadeh

Affiliations

Soon will be listed here.

Abstract

We aimed to explore the lncRNA-miR-mRNA network in response to Lactobacillus acidophilus (L. acidophilus) consumption in rectal cancer patients. The candidate miRs were first taken from the GEO and TCGA databases. We constructed the lncRNA-miR-mRNA network using the high-throughput sequencing data. At last, we created a heatmap based on the experimental data to show the possible correlation of the selected targets. The expression levels of selected targets were measured in the samples of 107 rectal cancer patients undergoing placebo and probiotic consumption and 10 noncancerous subjects using Real-Time PCR. Our analysis revealed a group of differentially expressed 12 miRs and 11 lncRNAs, and 12 genes in rectal cancer patients. A significant expression increase of the selected tumor suppressor miRs, lncRNAs, and genes and a substantial expression decrease of the selected oncomiRs, onco-lncRNAs, and oncogenes were obtained after the probiotic consumption compared to the placebo group. There is a strong correlation between some network components, including miR-133b and IGF1 gene, miR-548ac and MSH2 gene, and miR-21 and SMAD4 gene. In rectal cancer patients, L. acidophilus consumption was associated with improved expression of the lncRNA-miR-mRNA network, which may provide novel monitoring and therapeutic approaches.

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References

Li Y, Zeng C, Hu J, Pan Y, Shan Y, Liu B . Long non-coding RNA-SNHG7 acts as a target of miR-34a to increase GALNT7 level and regulate PI3K/Akt/mTOR pathway in colorectal cancer progression. J Hematol Oncol. 2018; 11(1):89. PMC: 6029165. DOI: 10.1186/s13045-018-0632-2. View

Rodriguez-Nogales A, Algieri F, Garrido-Mesa J, Vezza T, Utrilla M, Chueca N . Differential intestinal anti-inflammatory effects of Lactobacillus fermentum and Lactobacillus salivarius in DSS mouse colitis: impact on microRNAs expression and microbiota composition. Mol Nutr Food Res. 2017; 61(11). DOI: 10.1002/mnfr.201700144. View

Dalmasso G, Nguyen H, Yan Y, Laroui H, Charania M, Ayyadurai S . Microbiota modulate host gene expression via microRNAs. PLoS One. 2011; 6(4):e19293. PMC: 3084815. DOI: 10.1371/journal.pone.0019293. View

Ho C, Tan H, Chua K, Kang A, Lim K, Ling K . Author Correction: Engineered commensal microbes for diet-mediated colorectal-cancer chemoprevention. Nat Biomed Eng. 2020; 4(7):754-755. DOI: 10.1038/s41551-020-0580-3. View

Wang T, Cai G, Qiu Y, Fei N, Zhang M, Pang X . Structural segregation of gut microbiota between colorectal cancer patients and healthy volunteers. ISME J. 2011; 6(2):320-9. PMC: 3260502. DOI: 10.1038/ismej.2011.109. View

Khalighfard S, Kalhori M, Amiriani T, Poorkhani A, Khori V, Esmati E . A systematic approach introduced novel targets in rectal cancer by considering miRNA/mRNA interactions in response to radiotherapy. Cancer Biomark. 2021; 33(1):97-110. DOI: 10.3233/CBM-210079. View

Bao Z, Yang Z, Huang Z, Zhou Y, Cui Q, Dong D . LncRNADisease 2.0: an updated database of long non-coding RNA-associated diseases. Nucleic Acids Res. 2018; 47(D1):D1034-D1037. PMC: 6324086. DOI: 10.1093/nar/gky905. View

Gianotti L, Morelli L, Galbiati F, Rocchetti S, Coppola S, Beneduce A . A randomized double-blind trial on perioperative administration of probiotics in colorectal cancer patients. World J Gastroenterol. 2010; 16(2):167-75. PMC: 2806554. DOI: 10.3748/wjg.v16.i2.167. View

Zaharuddin L, Mokhtar N, Muhammad Nawawi K, Ali R . A randomized double-blind placebo-controlled trial of probiotics in post-surgical colorectal cancer. BMC Gastroenterol. 2019; 19(1):131. PMC: 6657028. DOI: 10.1186/s12876-019-1047-4. View

10.

Carames C, Cristobal I, Moreno V, Puerto L, Moreno I, Rodriguez M . MicroRNA-21 predicts response to preoperative chemoradiotherapy in locally advanced rectal cancer. Int J Colorectal Dis. 2015; 30(7):899-906. DOI: 10.1007/s00384-015-2231-9. View

11.

Heydari Z, Rahaie M, Alizadeh A, Agah S, Khalighfard S, Bahmani S . Effects of Lactobacillus acidophilus and Bifidobacterium bifidum Probiotics on the Expression of MicroRNAs 135b, 26b, 18a and 155, and Their Involving Genes in Mice Colon Cancer. Probiotics Antimicrob Proteins. 2018; 11(4):1155-1162. DOI: 10.1007/s12602-018-9478-8. View

12.

Urbanska A, Bhathena J, Martoni C, Prakash S . Estimation of the potential antitumor activity of microencapsulated Lactobacillus acidophilus yogurt formulation in the attenuation of tumorigenesis in Apc(Min/+) mice. Dig Dis Sci. 2008; 54(2):264-73. DOI: 10.1007/s10620-008-0363-2. View

13.

Sarver A, Sarver A, Yuan C, Subramanian S . OMCD: OncomiR Cancer Database. BMC Cancer. 2018; 18(1):1223. PMC: 6282392. DOI: 10.1186/s12885-018-5085-z. View

14.

Tay Y, Rinn J, Pandolfi P . The multilayered complexity of ceRNA crosstalk and competition. Nature. 2014; 505(7483):344-52. PMC: 4113481. DOI: 10.1038/nature12986. View

15.

Cantile M, di Bonito M, Tracey de Bellis M, Botti G . Functional Interaction among lncRNA HOTAIR and MicroRNAs in Cancer and Other Human Diseases. Cancers (Basel). 2021; 13(3). PMC: 7867281. DOI: 10.3390/cancers13030570. View

16.

Shen Y, Han X, Wang J, Wang S, Yang H, Lu S . Prognostic impact of mutation profiling in patients with stage II and III colon cancer. Sci Rep. 2016; 6:24310. PMC: 4830995. DOI: 10.1038/srep24310. View

17.

Gao Y, Shang S, Guo S, Li X, Zhou H, Liu H . Lnc2Cancer 3.0: an updated resource for experimentally supported lncRNA/circRNA cancer associations and web tools based on RNA-seq and scRNA-seq data. Nucleic Acids Res. 2020; 49(D1):D1251-D1258. PMC: 7779028. DOI: 10.1093/nar/gkaa1006. View

18.

Wang Z, Guo M, Ai X, Cheng J, Huang Z, Li X . Identification of Potential Diagnostic and Prognostic Biomarkers for Colorectal Cancer Based on GEO and TCGA Databases. Front Genet. 2021; 11:602922. PMC: 7841465. DOI: 10.3389/fgene.2020.602922. View

19.

Kim Y, Kim J, Park S . High-throughput 16S rRNA gene sequencing reveals alterations of mouse intestinal microbiota after radiotherapy. Anaerobe. 2015; 33:1-7. DOI: 10.1016/j.anaerobe.2015.01.004. View

20.

Sung H, Ferlay J, Siegel R, Laversanne M, Soerjomataram I, Jemal A . Global Cancer Statistics 2020: GLOBOCAN Estimates of Incidence and Mortality Worldwide for 36 Cancers in 185 Countries. CA Cancer J Clin. 2021; 71(3):209-249. DOI: 10.3322/caac.21660. View