Structural Insights into the Design of Reversible Fluorescent Probes for Metallo-β-lactamases NDM-1, VIM-2, and IMP-1

Overview

Journal J Inorg Biochem

Specialty Biochemistry

Date 2022 Jun 2

PMID 35653820

Authors

Sky Price

Radhika Mehta

Dominique Tan

Abigail Hinojosa

Pei W Thomas

Tawanda Cummings

Walter Fast

Emily L Que

Affiliations

Soon will be listed here.

Abstract

Metallo-β-lactamases (MBLs) are enzymes that are capable of hydrolyzing most β-lactam antibiotics and all clinically relevant carbapenems. We developed a library of reversible fluorescent turn-on probes that are designed to directly bind to the dizinc active site of these enzymes and can be used to study their dynamic metalation state and enzyme-inhibitor interactions. Structure-function relationships with regards to inhibitory strength and fluorescence turn-on response were evaluated for three representative MBLs.

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